| Objective To determine the effects of Ligustrazine on prevent and treat intestinal adhesion,and to research its influence on the Expressions of IL-1,TNF-? and TGF-?.Methods Eighty four SD male rats were randomly assigned to four groups:normal group(n =21),sham operated group(n =21),model group(n = 21)and drug group(n =21).In the sham operated group,we opened abdominal cavity and stitched it.In the model group and drug group,all the rats were made into intestinal adhesive models.From the first day after operations,10ml solution with 0.1g ligustrazine were injected into abdominal cavity in the drug group,while 10 mldehydrated alcohol was injected in the other groups,The dosage of each group was same.seven rats of each group was killed on day2,day7 and day 14.Observed the intestinal adhesion by naked eye and classify the adhesion level's.The adhesion tissues were taken and observed under light microscope.The blood samples of each group were collected to determine the cytokine levels by ELISA.Results Compared with normal group and sham operated group,the intestinal adhesion of model group and drug group was signicantly decreased(P<0.01).Compared with model group,the intestinal adhesion of drug group was signicantly decreased(P<0.01).Normal group,sham operated group and drug group had obvious staistics significance with model group(P<0.01).The IL-1 level of model group had no obvious staistics significance with grug group on day2(P>0.05)but had obvious staistics significance on day 7and day 14(P<0.01).The TGF-? level of model group had obvious staistics significance with grug group on day2,day7 and day14(P<0.05 or P<0.01).The TNF-a level of model group had obvious staistics significance with grug group on day2,day7 and day14(P<0.01).Conclusions ligustrazine has obvious effects on preventing and treating postoperative intestinal adhesion,it can inhibit intestinal adhesion by reducing the level of IL-1?TNF-??TGF-p. |
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