Study On Preparation Technology Of Organic Calcium And Chelate Calcium From Mactra Veneriformis

Mactra veneriformis?family:Mactridae?,a typical marine bivalve mollusk,is a delicious sea food and a traditional Chinese medicine while being low-cost,ubiquitous and abundant in Chinese coastal areas,especially in the coastal shoals of Jiangsu province.The bioactive compounds such as carotenoids,lipids,polysaccharides were found in the flesh of Mactra veneriformis.During the initial research period,our team devoted to study the biological activity of water-soluble polysaccharide extracted from Mactra veneriformis,while majority of residual meal,which rich in protein was discarded.Therefore,Mactra veneriformis residual meal utilization contributes to environmental conservation as an energy saving resource,how to take advantage of scraps is particularly important.Following parts were mainly included:1.Literature studyResearch papers about calcium were referred,and summarized the progress of bivalve shells prepared for calcium preparations.Research papers about calcium function of calcium products were referred,and recent developments of shellfish zymolysing peptide and shell applications were understood.Thus literature foundation of further experiment operation was laid.2.The study of Mactra veneriformis shell preparation of calcium citrate process?1?Studying and establishment of Mactra veneriformis shell prepare for calcium citrate process,and get the secondary calcination preparation process,while using the Box-Behnken model response surface design,the preparation of calcium citrate ternary quadratic regression orthogonal model.The results to determine the optimal preparation conditions for citric acid/calcium as 2.78,liquid/calcium as 6.11,the reaction time of 1.9h,the reaction temperature of 60?,stirring time of 10min.The end of calcium citrate content was 99.02%,and yield was 91.78%.?2?Through the analysis of the quality of the product of calcium citrate,calcium citrate prepared in a large amount of money a lead content of 0.0002%arsenic content is less than 0.00006%;loss on drying and other experiments were qualified,and this prepared lemon calcium contentup to 98.6%,in line with the requirements of GB calcium citrate.?3?Prescription compatibility of the preparation of the calcium citrate to study the composition and amount of excipients,finalized add VB2 0.02%,VD3 0.000125%,microcrystalline cellulose 8%,mannitol 12%,stearic acidmagnesium 1%,0.2%as aspartame.?4?Process of the preparation of calcium citrate enlarge pilot study provides a theoretical basis for future industrial production and reliable test parameters.The advantage of the process of the traditional preparation,calcium citrate was prepared by the Mactra veneriformis shells,good purity,high yield,in full compliance with food standards,and no heavy metal pollution.3.The study of Mactra veneriformis enzymatic peptide chelated calcium?1?Evaluation of the different conditions of the hydrolysis process,and preparation to determine the ability of chelating calcium activity of the Mactra veneriformis enzymatic peptide hydrolysis process conditions:pepsin as a hydrolytic enzyme with pH 2.5,temperature 37?,time 7h,enzyme-substrate ratio of 1.5%.?2?Investigated on ovariectomized rats the supply of the enzymatic peptide significantly improve their calcium deficiency caused by serum biochemical indices and femur growth index,showed that the Mactra veneriformis enzymatic peptide heavy ashes,ovariectomized rat serum biochemical indicators of stock shares bone mineral density,femur resistance to bending capacity,osteocalcin and bone phosphorus,toward the development of more conducive to the direction of the femoral growth.?3?Examined the Mactra veneriformis prepared by the hydrolysis of peptides with calcium chelating peptide chelate calcium process conditions,optimum conditions are as follows:calcium ratio was 2.5:1 CaCl₂ solid,adjusted to pH 6.0,40 ? water bathing for 2.5 hours.Obtained in optimum conditions,peptide chelated calcium in the calcium content of 4.69%,the ultraviolet and infrared spectroscopy,and further define the Mactra veneriformis peptide chelating calcium.