Silencing Of 5SrRNA By MiR-150 And MiR-383 Leads To Growth Arrest In Esophageal Squamous Cancer

Esophageal cancer is a common malignant of gastrointestinal tract with a poor prognosis, it is the sixth most common cancer worldwide, It has two main forms which include esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (ADC). its incidence rate and mortality rate show a significant geographic difference around the world, owing to its poor prognosis it is important to find the exact mechanism to solve many problems existing in the esophageal cancer.We focus the research on 5SrRNA, it is transcribed by RNA polIII, a main component of the large ribosomal subunit, which stabilizes the structure of the large ribosomal subunit. Reports show that abnormal ribosome synthesis and protein translation appears to be associated with the biological processes of tumor. Besides its role in ribosome assembly, several recent studies have revealed that 5SrRNA also plays a role in sensing ribosomal stress by forming 5S-RPL5-RPL11 complex. It is important to regulate 5SrRNA particularly and exactly in cell growth and proliferation.We first found that overexpression of miR-150 and miR-383 could lead to down-regulation of 5SrRNA in a series of esophageal squamous cancer cells in this article, we also found that the cell numbers which transfected miR-150 and miR-383 significantly reduce compared with Negative control, which revealmiR-150 and miR-383 inhibit cell proliferation through down-regulation of 5SrRNA.Overexpression of miR-150 and miR-383 induce p21 expression and inhibit KYSE510 cell growth in a p53 independent pathway. The relevant results not only shed light on the post-transcriptional regulation of 5SrRNA but also provide potential therapeutic strategies for ESCC that harbor inactive form of p53.