Research Of The Severe Hand, Foot And Mouth Disease Differentially Expressed Genes Based On The Rna Sequencing Technology

Objective To screen severe hand, foot and mouth disease(SHFMD) differentially expressed genes(DEGs), and for provide theoretical basis for the early identification and timely treatment of SHFMD by high-throughput transcriptome sequencing technology(RNA sequencing, RNA-seq). And to reduce the mortality and improve the prognosis of the disease.Methods A total of 15 PBMC samples were collected from 5 SHFMD patients, 5 MHFMD(mild hand, foot and mouth disease, MHFMD) patients and 5 healthy control(HC), during May 2014 and August 2014, in the third people's hospital of shenzhen. Using the RNA-seq technology we sequenced the RNA of the PBMC, on the basis of RPKM(Reads Per Kilobase of exon model per Million mapped reads) multiples and expression level fold-change, and screened the differentially expressed genes. Furthermore, we confirmed the expression levels of the DEGs by quantitative real-time PCR(q RT-PCR) and enzyme-linked immunosorbent assay(ELISA).Results RNA-seq found 117 DEGs between SHFMD and MHFMD, including 108 up-regulated genes and 9 down-regulated genes; and 26 DEGs between SHFMD and HC, including 14 up-regulated genes and 12 down-regulated genes. The two groups of DEGs had 8 genes in common, of which S100A8, S100A12, IL-8 and IL-1? were up-regulated, while TNFRSF13 C, IL-7R, THBS1 and VEGFA were down-regulated. And q RT-PCR and ELISA detection results are consistent with the results of RNA-seq(P<0.05).Conclusions 1.RNA-seq technology successfully establishment of gene expression profiles of HFMD. 2.Up-regulation of S100A8, S100A12, IL-8, IL-1? and down-regulated TNFRSF13 C, IL-7R, THBS1, VEGFA were SHFMD differentially expressed genes, is expected to become the predictor of severe HFMD.