The Expression And Clinical Significance Of CUL4A And NF-?B P65 In Pancreatic Cancer

Objective:CUL4A plays a key role in the process of the ubiquitination, a many of studies have indicated that CUL4 A is a new oncogene. This study is to investigate the expression of NF-?B p65 and CUL4 A in pancreatic cancer tissues and normal pancreatic tissues and to analyze the relationship between CUL4 A and NF-?B p65 in pancreatic cancer differentiation, staging and lymph node metastasis by immunohistochemistry. Our aim is to further researching the possible mechanism and clinical significance of CUL4 A and NF-?B p65 in the occurrence, development and metastasis of pancreatic cancer,so as to lay the foundation for the further development of pancreatic cancer.Methods: 43 cases of pancreatic cancer paraffin block tissues and 12 cases of normal pancreatic tissues,which are greater than 2cm from tumor tissues,were collected from 2010 to 2015 in Chenzhou First People's Hospital. All of these tissues were confirmed pancreatic cancer by surgery and preoperative chemotherapy and radiation therapy were not performed in these patients. The expression of NF-?B p65 and CUL4 A in pancreatic cancer tissues and normal pancreatic tissues was detected by immunohistochemistry(SP method). The experimental data was analyzed by chi square statistical software of SPSS19.0 for the relevance CUL4 A and NF-?B p65 in pancreatic cancer cases(including gender, age, tumor size, clinical stage, differentiation degree, lymph node metastasis). Correlation between CUL4 A and NF-?B p65 in pancreatic cancer tissues was analyzed by Spearman correlation analysis, P < 0.05 indicating that there was statistical significance.Results:(1)The positive rate of CUL4 A in 43 cases of pancreatic cancer tissues was 74.4%(32 / 43). The positive rate of CUL4 A in 12 cases of normal pancreatic tissues was 33.3%(4 / 12),?2=7.004, P = 0.008 < 0.05, indicating a statistically significant. NF-?B p65,s positive rate was 65.1%(28 / 42) in 43 cases of pancreatic cancer tissues and 25.0%(3 / 12)in 12 cases of normal pancreatic tissues, ?2=6.139, P = 0.013 < 0.05, indicating a statistically significant.(2)The correlation between CUL4 A and clinical pathology of 43 cases of pancreatic cancers: their was no relationship between CUL4 A and gender, age, tumor size, P > 0.05,but was relationship between CUL4 A and tumor differentiation, TNM stage, lymph node metastasis, P <0.05,indicating that there was statistical significance.(3)The correlation between NF-?B p65 and clinical pathological data of 43 cases of pancreatic cancers: their was no relationship between NF-?B p65 and gender, age, tumor size, P > 0.05,but was relationship between NF-?B p65 and tumor differentiation, TNM stage, lymph node metastasis, P <0.05,indicating that there was statistical significance.(4)The correlation of expression between CUL4 A and NF-?B p65 in pancreatic cancer tissues: There were 25 cases of NF-?B p65 positive expression in 32 cases of CUL4 A positive expression. There were 8 cases of NF-?B p65 negative expression in 11 cases of CUL4 A negative expression. The expression between CUL4 A and NF-?B p65 in pancreatic cancer tissues was positively related, r=0.466, p=0.002, indicating that there was statistical significance.Conclusion:(1)The expression of CUL4 A and NF-?B p65 in pancreatic cancer tissues was higher than normal pancreatic tissues,which indicating that the occurrence of pancreatic cancer may be related to NF-?B p65 and CUL4 A.(2)The expression of CUL4 A and NF-?B p65 in pancreatic cancer tissues with low differentiation degree, TNM high stage and lymph node metastasis were more obvious than mormal pancratic tissues, which indicating that the development and metastasis of pancreatic cancer may be related to CUL4 A and NF-?B p65.(3)CUL4A and NF-?B p65,s expression in pancreatic cancer tissues was positively related,which indicating that the expression of CUL4 A may activate the NF-?B signaling pathway,then lead to Epithelial mesenchymal cell transformation,then promoting the Occurrence, development and metastasis of pancreatic cancer.