The T-cell Immunological Properties Of Tuberculosis Latent Protein Rv2659c In Active Bone Tuberculosis

Approximately one third of the world's population is infected wi th M.tb, 9 million develop TB disease and 1.8 million die annually. The success of Mycobacterium tuberculosis(M.tb) as a human path ogen may be ascribed to its ability to persist for long periods in as ymptomatic people, a state known as latency. Healthy latently infect ed individuals have approximately 10% lifetime risk of developing ac tive TB disease. In recent years, with the ongoing study of latent t uberculosis infection, some scholars found that differences of the ho st microenvironment can change the gene expression profile of M.tb.Researchers simulate a nutritionally compromised environment, acce ss to a large number of M.tb transcription data, discovered several nutritional deficiencies associated latency antigen. Latent infection rel ated protein Rv2659 c induced by high levels of T cell immune respo nse in latent tuberculosis infection, however, the t-cell-mediated immu ne response is not clear in active bone tuberculosis. 3333333IFN?? pr oduction from the peripheral blood mononuclear cell(PBMC) was ass ayed by enzyme?linked immunospot assay(ELISPOT) among healthy v olunteers who consisted of uninfected healthy subjects(42 cases) an d those with latent tuberculosis patients(LTBI)(33 cases).Non?tubercu losis bone disease patients(35 cases) and active bone?joint tuberculo sis patients(30 cases) were filtered out among the inpatient volunte ers.33333 33333333333 3333333333 To draw Rv2659 c protein of a ROC curve, the SFC values stimulated by antigen of LTBI subjects we re taken as the positive group, and the active bone tuberculosis pat ients were used as a control group. Using the cut?off values to eval uate the performance of Rv2659 c in differential diagnosis of LTBI an d active bone tuberculosis. Using non?parametric tests to analysis da ta. Analyzing non?parametric tests: when stimulated by Rv2659 c, PB MC from latent infection group(2.5/28) yielded significantly higher ELISPOT counts than those from active bone tuberculosis patients(0/2), non?tuberculosis of bone disease patients(2.5/6.25) and uninfe cted healthy subjects(0/2.5)(p <0.05). Analysising ROC curve: The a rea under the curve of Rv2659 c was up to 65.2% and the 95 confi dence interval was 0.51?0.794. When the specificity is 80%, the Cut?off value is 3; specificity is 90%, the Cut?off value is 5.When stimul ated by Rv2659 c, the positive rate of latent infection(54.5%) was h igher than that among active bone tuberculosis patients(20%), non?tuberculosis of bone disease patients(11.4%)and uninfected healthy subjects(16.7%)(p<0.05). These studies indicate that Rv2659 c is iden tified difficultly by active bone tuberculosis patients and induced lo wer immune response via effector T cells compared with LTBI subje cts;Rv2659c protein shows good performance in active bone tuberc ulosis differentiation and latent TB identification.