The Protective Role Of Sulforaphane In Oxidative Stress Damage On Bovine Trabecular Meshwork Cells Induced By Hydrogen Oxygen

ObjectiveTo investigate the antioxidant effect of sulforaphane (SFN) against H2O2-induced oxidative damage in bovine trabecular meshwork (BTM) cells.MethodsBovine trabecular meshwork cells were exposed to H2O2 to induce oxidative damage. Treatment with different dilutions of sulforaphane, and test cell viability at 6h,12h,24h, to explore the best interfere concentration of sulforaphane and time length. Pretreatment with adequate concentration of SFN was performed 6h before exposure to H2O2.then cell viability and apoptosis rate was conducted by CCK-8 and Annexin-V FITC/PI staining flow cytometry. ResultsThe damage induced by 200μ mol·L-1/and 300μ mol·L-1H2O2 to trabecular meshwork was too serious, whereas 100μmol·L-1/H2O2 not only can induce adequate oxidative damage but also can allow sulforaphane exhibit its protective role. Cell viability is not significant different between 10μmol·L-1 SFN and the control group at 6 hour. However, other concentration (20μ mol·L-1,30μmol·L-1 40μmol·L-1 of SFN can decrease cell ability. Pretreatment with 10μ mol·L-1 SFN for 6h, cells viability significant higher and apoptosisi rate lower after followed by 100μmol·L-1 H2O2 for 6h,in contrast to the H2O2 group.ConclusionsSulforaphane may improve antioxidative stress ability of trabecular meshwork cell, and decrease the damage induced by oxidative stress.