The Expression Of Urinary Exosomal MiR-193a In Idiopathic Membranous Nephropathy Patients

Objective: Idiopathic membranous nephropathy(IMN)is one of the most common diseases,which can usually cause primary glomerulonephritiseven nephritic syndrome.Alleviation spontaneously can be found in Some patients;but more than a half patients could found changes of renal function or uremia.Therefore,it has a great significant of researching the pathogenesis of the IMN.MicroRNA(miRNA)is a small non-coding RNA molecule(containing about 22~25 nucleotides)that functions in RNA silencing and post-transcriptional regulation of gene expression.In recent years,The miRNAs have been gradually used in the research of the renal disease.In some researchs,founded that Wilms' tumor protein(WT1),a transcription factor and master regulator of podocyte differentiation and homeostasis.Decreased expression levels of WT1 lead to downregulation of its target genes PODXL(podocalyxin)and NPHS1(nephrin),as well as several other genes crucial for the architecture of podocytes,initiating a catastrophic collapse of the entire podocyte-stabilizing system.We founded upregulation of miR-193 a in isolated glomeruli from individuals with FSGS compared to normal kidneys or individuals with other glomerular diseases.Thus,upregulation of mi R-193 a provides a new pathogenic mechanism for FSGS and is a potential therapeutic target.Both IMN and FSGS are defined as mycetocyte,thus,we can speculate that IMN patients with massive proteinuria also have the increase expressions of the miRNA-193 a.And after alleviation the expressions may decline.In some research,exosomes in the urine can display the physiological or pathological status of the whole urinary system.What is more,the exosomes contain all the information and is an ideal marker.In this research,we extract the miRNA from urinary exosomes which is from the IMN patients,to observe the miR-193 a expressing changes,and to settle a basement of researching the occurrence,development mechanism of IMN.Method :a.Subject : We enrolled 42 patients(IMN NS group 14,IMN remission group 14,normal control group 14)with the matched age and gender.b.Measurement of miR-193a: We choosed Exoquick kits to extract the exosomes in the urine,and plant based mi R-168 a was used as a reference gene.After that extract the RNA from the exosomes by using the Trizol kits.Finally,we tested the molecules expressions of the miR-193 a by qRT-PCR.Result:The miRNA-193 a expression of urine exosome in idiopathic membranous nephropathy patients was much higher than that in healthy controls(the relative expression ratio was 4.30 ± 0.66 vs 1,P < 0.05).Meanwhile,the urine exosomal miRNA-193 a expression in nehprotic IMN patients was significantly higher than that in complete or partial remission IMN patients(the relative expression ratio was 6.07 ± 0.72 vs 2.54 ± 0.43,P < 0.05).Conclusion: The miRNA-193 a expression of urine exosome in idiopathic membranous nephropathy patients was much higher than that in healthy controls.The expression was downregulated as proteinuria remission.Our results suggested that miRNA-193 a might play an important role in the pathogenesis of IMN.