Effects Of IL-17 On Apoptosis And Oxidative Stress Of Cementoblast In Vitro

Objective:The study aims to investigate the influence of different concentration of Interleukin 17(IL-17A)on apoptosis and oxidative stress of cementoblast in vitro.In this study,we investigate the role of IL-17 A on root resorption and provide evidence for preventing root resorption in clinical work.Method:Cementoblast cell line OCCM-30 were cultured at 37 ? in a humidified atmosphere of 5%CO2 and were divided into experimental and control groups with or without IL-17 A.The control group was applied with DMEM culture medium supplemented with 10% FBS.Therefore the experimental groups were intervened by IL-17 A at the concentration of 10ng/ml?20 ng/ml?30 ng/ml?40 ng/ml?50 ng/ml.1.The proliferation of cementoblast was evaluated by CCK-8 analysis on the 24h?48h?72h after the intervention of IL-17 A.2.The apoptosis of cementoblast was measured by Flow Cytometry(FCM)after exposed to IL-17 A for 48 h.3.The level of ROS was measured by FCM after exposed to IL-17 A for 48 h.Results:1.The proliferation of cementoblast measured by CCK-8OCCM-30 viability was significantly inhibited by IL-17 A.Compare with the control group,the OD value of experimental groups after exposed to IL-17 A for 48h?72h was gradually increase,there was statistically significant difference(P<0.01).But after exposed to IL-17 A for 24 h,the OD value of experimental groups was no significant difference(P>0.05).2.The apoptosis of cementoblast measured by FCMAfter exposed to IL-17 A for 48 h,the early and late apoptosis rate of experimental groups were remarkable elevated with the rising of concentrationcompared with control group,which was significant statistically difference(P<0.01).3.The ROS level of cementoblast measured by FCMThe ROS level of experimental group supplemented with 10ng/ml IL-17 A was increased in 48 h compared with the cntrol group,there was significant statistically difference(P<0.05);and the ROS level of 20 ? 30 ? 40 ? 50ng/ml group were conspicuously increased,which was significant statistically difference(P<0.01).Conclusion:1.At the concentration of 10-50ng/ml IL-17 A can decrease the proliferation of OCCM-30,which is concentration-dependent.2.At the concentration of 10-50ng/ml IL-17 A can promote apoptosis of OCCM-30,and the effect is concentration-dependent.3.IL-17 A can promote ROS production and oxidative stress response in cementoblast,and the effect is dose-dependent.In addition,IL-17 A may exerted a negative effect on proliferation of cementoblast through promoting ROS production and oxidative stress response in cell.