The Experiment Research Of Inhibitory Scarring Effect Of Pirfenidone On Rabbit Glaucoma Filtrating Surgery

Objective: By the experiment research of inhibitory scarring effect of pirfenidone on rabbit glaucoma filtrating surgery,to further provide basis for the effect of pirfenidone.Methods: 1.laboratory animal:28 healthy adult new Zealand rabbit,quality2.0Kg-2.5Kg,4 only randomly as the blank group,another 24 rabbits(24 eyes,right eye)was divided into 3 groups: experimental group ? positive control group ? negative control group,every group has 8 rabbits.Glaucoma filtering surgery with pirfenidone on sclera flap intraoperative and conjunctival flap after surgery was conducted in the experimental group.Glaucoma filtering surgery combined with MMC in positive control group.Glaucoma filtering surgery combined with normal saline(NS)in negative control group.2.observation method:Observed the IOP and filtering bleb in 1,7,14,28 days after surgery,and put rabbits on death in 1,2,4,12 weeks after surgery.Then extirpated eyeballs of the rabbits.HE staining and immune-histochemical staining and Western Blot were made to compare ?-SMA?VEGF?TGF-?1.Result: 1.Postoperative 1 week,all eyes were conjunctival congestion and secretion,the experimental group did not appear anterior segment injury,positive control group has a corneal epithelial dot defect and anterior chamber fibrin exudation,negative control group has anterior chamber bleeding,all went away in 1 week after surgery.2.The IOP of all groups dropped significantly in early postoperative.With the extension of the time,the IOP increased.The IOP of the negative group and experimental group,the IOP of the negative group and positive control group have obvious difference(P<0.05).No significant difference between experimental group and positive control group(P > 0.05).The experimental group and the positive controls have more functional filtering bleb than the negative group,the difference was statistically significant(P < 0.05).3.At 1 week after surgery,?-SMA?VEGF?TGF-?1 are expressed in each group,and expression of the negative group were stronger than the other two groups.At 1 month after surgery,the expression of 3 groups were reduced,but the expression of negative group is significantly stronger than the experimental group and positive control group,the difference was statistically significant(P < 0.05)Conclusion: 1.pirfenidone used in glaucoma filtrating surgery area under the sclera flap and conjunctival flap can inhibit ?-SMA?VEGF?TGF-?1,thus inhibiting filter corridor scar hyperplasia,maintain ideal postoperative IOP.2.pirfenidone can inhibit the expression of VEGF,reduce the filtration area new blood vessels.3.Pirfenidone is nonirritating and safty.