| Background and ObjectiveSpinal cord injury is a serious injury of central nervous system, caused by a variety of reasons. It has been reported that spinal cord injury patients who lead to motor and sensory function independently were between 11.5 million and 53.4million in the world each year. Spinal cord injury can be divided into primary spinal cord injury and secondary spinal cord injury caused by primary factor. Primary spinal cord injury refers to damage directly caused by the injury factors in a short time after spinal cord injury. It is a irreversible damage. It is the main factor leading to secondary spinal cord injury which a large number of inflammatory cell infiltration and a large number of inflammatory cytokine production after primary spinal cord injury. Because of its reversibility, the control of secondary spinal cord injury is an important goal of early treatment in acute spinal cord injury. Melatonin is an amphoteric molecule and it is extracted in the pineal gland. There is evidence that melatonin has neuroprotective, reducing oxidative stress, reducing edema and so on.In recent years, Chinese and foreign scholars were explored polymorphonuclear leukocyte, cytokine-induced neutrophil chemoattractant-1, intercellular adhesion molecule-1, interleukin-1β after acute spinal cord injury in different ways. The correlation between melatonin and them are mostly not studied. In this study, we used melatonin after acute spinal cord injury, to observe their expression in different times.Material and MethodsThe SD rats(108) break randomly into three groups: blank control group(A group,n=36)、 spinal cord injury group(B group,n=36)、MT treatment group(C group,n=36). B and C groups were established the SCI animal models at T11 level.Lamina of vertebra was cut without spinal cord injury in the blank control group. The rats of B group were injected absolute ethyl alcohol(the solvent of MT) into abdominal cavity after spinal cord injury while the rats of C group were injected MT(100mg/kg). Fetching the spinal tissue at 1h, 6h, 12 h, 1d, 3d and 5d after spinal cord injury. Chemical method was used to detect the expression of MPO. The hematoxylin-eosin staining was performed to the spinal cord to observed thepathological changes in the groups respectively. RT-PCR was used to detect the expression of CINC-1、IL-1β in spinal cord. Immunohistochemical was used to detect the expression of ICAM-1 in spinal cord. Statistical analysis was performed with the SPSS 19.0 software. All values were expressed as mean standard deviation. Statistical analysis of data was performed using a one-way analysis of variance( ANOVA).Statistical significance was assumed for P < 0.05.Results1. Blank control group has a small expression of IL-1β. Spinal cord injury group has a significant increase and peaked at 6h after spinal cord injury. After spinal cord injury 6h、12h、1d、3d、5d, the expression of IL-1β in MT treatment group was significantly lower than that in spinal cord injury group.2. Blank control group has a small expression of CINC-1. Spinal cord injury group has a significant increase and peaked at 12 h after spinal cord injury. After spinal cord injury 6h、12h、1d、3d、5d, the expression of CINC-1 in MT treatment group was significantly lower than that in spinal cord injury group.3. Blank control group has a small expression of ICAM-1. Spinal cord injury group has a significant increase and peaked at 12 h after spinal cord injury. After spinal cord injury 6h、12h、1d、3d, the expression of ICAM-1 in MT treatment group was significantly lower than that in spinal cord injury group.4. MPO has a low expression in blank control group and no significant change in each time point. Spinal cord injury group has a significant increase. It peaked at 1d after spinal cord injury. After spinal cord injury 6h、12h、1d、3d、5d, the expression of MPO in MT treatment group was significantly lower than that in spinal cord injury group.ConclusionMelatonin can reduce the infiltration of PMNL, inhibit expression of CINC-1、ICAM-1 and IL-1β, reduce the inflammatory response after spinal cord injury. |
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