The Effect Of HGC-MSCs On The Tumor Growth Of Gastric Cancer-Bearing Mice

Objective To investigate the effect of hGC-MSCs on the tumor growth of gastric cancer-bearing mice.Methods Gastric cancer cell line SGC-7901 were cultured and inoculated subcutaneously in the right back of BABL/c nude mice with a number concentration of 1×107 for each one to form the tumor-bearing mice model. The tumor-bearing mice receiving different doses (0.5 X 106,1.0×106, 2.0 X 106) of hGC-MSCs were classified into the experimental groups, while those receiving PBS were taken as the control group. The changes in tumor volume were measured, the expression of PCNA and MMP-9 by immunohistochemistry?real-time PCRand Western blot in each group was detected.Results The tumor volume in the experimental groups was significantly larger than that of the control group, It is statistically significant difference (P<0.05). The immunohistochemistry results showed that the average optical density of PCNA in the control group and experimental group was (0.349±0.008), (0.376±0.013), (0.441±0.012) and (0.5±0.022), respectively; the average optical density of MMP-9 in these four groups was (0.341±0.027), (0.429±0.015), (0.474±0.006) and (0.527±0.033), respectively, It is statistically significant difference (P<0.01). Real-time PCR demonstrated that hGC-MSCs can upregulate the expression m-RNA of PCNA and MMP-9 (P<0.05). Western blot revealed the expression of PCNA in the control group and the experimental group (14.99±0.9), (17.64±0.9), (25.11±0.4) and (28.31±1.1), respectively; the MMP-9 expression in these four groups was (18.48±1.3), (32.95±1.2), (67.26±1.2) and (141.16±3.8), respectively, It is statistically significant difference (P<0.01).Conclusion hGC-MSCs can promote the tumor growth of gastric cancer-bearing mice in vivo, and its mechanism may be associated with the upregulated expression of PCNA and MMP-9.Objective To investigate the effect of hGC-MSCs on the tumor formation of gastric cancer cell line SGC-7901.Methods The nude mice were divided into SGC-7901 group, hGC-MSCs group and hGC-MSCs+ SGC-7901 group. The corresponding cell suspensions were inoculated subcutaneously in the right back of BABL/c nude mice. Then the presence of tumor and time were observed, and the changes in tumor volume, immunohistochemistry and real-time PCR were measured; the expression of PCNA and MMP-9 by Western blot in each group was detected.Results hGC-MSCs group showed no tumor formation; the tumor formation time of hGC-MSCs +SGC-7901 was significantly shorter than that of SGC-7901 group, with faster tumor growth and larger volume (P<0.01). The immunohistochemistry results showed that the average optical density of PCNA in SGC-7901 group and hGC-MSCs+SGC-7901 group was 0.358±0.009 and 0.481± 0.030, respectively; the average optical density of MMP-9 in these two groups was 0.359+0.033 and 0.520+0.038, respectively, with a statistically significant difference (P<0.05). Real-time PCR demonstrated that hGC-MSCs can upregulate the expression of PCNA and MMP-9 (P<0.01). The PCNA protein band grayscale in SGC-7901 group and hGC-MSCs+SGC-7901 group detected by Western blot was 0.756±0.039 and 0.847±0.048, respectively; the MMP-9 protein band grayscale in these two groups was 0.692±0.079 and 0.866±0.010, respectively, with a statistically significant difference (P<0.05).Conclusion hGC-MSCs can promote the tumor formation and growth in vivo, and its mechanism may be associated with the upregulated expression of PCNA and MMP-9.