The Role Of Urine In Prostatic Urethra Re-epithelialization After Prostatectomy

Objective:To explore the effect of urine in prostatic urethra re-epithelialization after prostatectomy.Methods:Two types of TmLRP animal models were established.One type was with urine simulation,the other one without urine simulation (ureterocutaneostomy and cystostomy). Then to detect the wounds by cystoscopy and immunohistochemistry after 1,3 and 4 weeks respectively. BPH-1 cell lines were cultured with or without urine and the proliferation and migration were tested by CCK-8 and Transwell assays. Results:Cystoscope test showed that no regenerated epithelium was observed in both groups after 1 week. After 3 weeks, the regeneration epithelium covered almost all of the wounds in urine simulation animal models. On the contrast, no regenerated epithelium was found in models that were without urine stimulation. After 4 weeks, the re-epithelialization was completed in the models that were stimulated by urine, while in other models that were without regenerated epithelium, there was still some wound spots in the counterpart. The results of immunohistochemistry showed that the migration, budding and extending to the wound were found in urine stimulating animal models, but no germinated epithelialization was found in the models that were without urine stimulate after 1 week. After 3 weeks, the umbrella cells and more layers of regeneration were found in urine stimulating animal models. Yet, some spots of wound were not covered at this moment in no urine stimulating animal models. After 4 weeks, germinating epithelium fully covered the wound in urine stimulating animal models. However, some small focus were still without regeneration in other group animal models. In CCK-8 and Transwell assays, an increased cell proliferation and migration were detected in the group which was under the effect of urine than other group that were without stimulation of urine (P<0.05). Conclusion:Urine may speed up the process of the prostatic urethra re-epithelialization by promoting the basal cells proliferation and migration.