The Implication Of TRIM13 On Autophagy In HEK293T Cells

Objective: The TRIM(tripartite motif)family of proteins is characterized by the presence of the tripartite motif module.A large number of studies have indicated that TRIM protein is involved in cell apoptosis.?eurodegenerative disease?Body immune response ? Regulation of tumor growth and Cellular physiological processes of cell response to virus.In the present paper,there has found the close relationship between the TRIM family and disease that HIV virus infection,leukemia,Parkinson's disease,Louis' s disease and lung cancer.It can degradation and recovery of damaged organelles and other cellular debris.It is a cellular protective process,which can prevent infection,cancer,cell aging and neurodegenerative disease and other pathological conditions.Autophagy,which could clear the aggregation or defect of the mutant proteins,could cause the diseases such as Huntington's,Perkins' s,amyotrophic lateral sclerosis,Alzheimer's disease,and diabetes.It is helpful for studying autophagy to understand the development of the disease.TRIM13,also known as RFP2,is a member of the TRIM family,which is localized in the endoplasmic reticulum.We have little known about the cellular functions of TRIM13.In the recently paper,TRIM13 has relationship in degradation of intracellular proteins and degenerative neuropathy.Weneeds more investigation to study these diseases.In this paper,in order to provide more reference for the functional analysis of TRIM13 and the mechanism of TRIM protein related diseases,we study the relationship between TRIM13 and autophagy.Methods:(1)We induce HEK293 T cells by rapamycin to establish the autophagy cell model.(2)We use the PCR real-time and Western blot to detect the gene and protein expression of TRIM13 and LC3-II in the autophagy group and control group.(3)We down-regulate TRIM13 specifically to establish the autophagy knockout cell model.(4)We use the PCR real-time and Western blot to detect the gene and protein expression of LC3-II in the autophagy negative group,autophagy blank group and autophagy knockout group.Results:(1)To compared with the control group,the expression of LC3-II gene and protein in autophagy cell group was significantly increased(P < 0.05).(2)To compared with the control group,the expression of TRIM13 gene and protein in autophagy cell group was significantly lower(P < 0.05).(3)To compared with the autophagy negative group and autophagy blank group,the LC3-II gene and protein expression in autophagy knockout group were significantly different(P < 0.05).The degree of autophagy was decreased in the autophagy knockout group.Conclusions:(1)the content of TRIM13 in autophagy cells was significantly lower than that in normal cells.(2)TRIM13 is involved in HEK293 T autophagy,which is likely to be consumed in the process of autophagy.