The Expression Of FBXO44 And Its Relationship With RAS,mt-P53 In Breast Cancer

Objective:To investigate the expression of FBXO44,RAS and mt-P53 in noncancerous breast ductal epithelial cells,DCIS cells and IDC cells of sparaffin-embedded specimens and the expression of FBXO44 in the normal breast ductal epithelial cell line and the breast cancer cell lines.To discuss the relationship between the expression of FBXO44,RAS and mt-P53 in breast cancer and the relationship between the FBXO44'expression and clinical pathologic characteristics.Methods:1.To detect the expression of FBXO44,RAS and mt-P53 by Envision immunohistochemistry in normal breast ductal epithelial cells of 32 cases,benign disease ductal epithelial cells of 32 cases,DCIS cells of 32 cases and IDC cells of 32 cases.2.To detect the expression of FBXO44 by Western Blot in the normal breast ductal epithelial cell line(HBL-100)and the breast cancer cell lines(MCF-7,MDA-MB-231).Results:1.The positive rate of FBXO44 in normal breast ductal epithelial cells,benign disease ductal epithelial cells,DCIS cells and IDC cells was 3.1%(1/32),12.5%(4/32),50.0%(16/32)and 78.1%(25/32)separately.The difference between benign disease and normal breast tissue had no statistical significance(P>0.05),while the difference between DCIS and benign disease,IDC and benign disease had obviously statistical significance(P<0.01).The difference between IDC and DCIS was statistically significant(P<0.05).The expression of FBXO44 was associated with ER and axillary lymph node metastasis(P<0.05).2.RAS was not expressed in normal breast ductal epithelial cells.The positive rate of RAS in benign disease ductal epithelial cells,DCIS cells and IDC cells was 21.9%(7/32),62.5%(20/32)and 71.9%(23/32)separately.The differences between DCIS and IDC had no statistical significance(P>0.05),while the difference between benign disease and normal breast tissue had statistical significance(P<0.05),the difference between DCIS and benign disease,IDC and benign disease had obviously statistical significance(P<0.01).The expression of RAS was correlated with histological grade(P<0.05).3.The positive rate of mt-P53 in normal breast ductal epithelial cells,benign diseaseductal epithelial cells,DCIS cells and IDC cells separately was 9.4%(3/32),15.6%(5/3),21.9%(7/32)and 56.3%(18/32).The difference between benign disease and normal breast tissue,DCIS and benign disease had no statistical significance(P>0.05),while the difference between benign disease and IDC,DCIS and IDC had obviously statistical significance(P<0.01).The expression of mt-P53 was related to axillary lymph node metastasis(P<0.05).4.The positive correlation was found between the expression of FBXO44 and that of mt-P53(r=0.611,P<0.01).5.The expression of FBXO44 in the breast cancer cell line MDA-MB-231(0.751±0.012)was significantly higher than that of in the normal breast ductal epithelial cell line HBL-100(0.412±0.015,P<0.01).Conclusion:1.The expression of FBXO44 in IDC and DCIS was significantly higher than that of in benign disease and normal breast tissue,and the FBXO44'expression in IDC is also higher than that of in DCIS.It suggests that FBXO44 may have a catalytic role in the development of breast cancer.2.The expression of FBXO44 was correlated with ER and axillary lymph node metastasis.FBXO44 may be involved in the metastasis and prognosis of breast cancer.3.The expression of FBXO44 had positively related with mt-P53,indicating that detecting both of them may contribute to predict the biological behavior of breast cancer.4.The expression of FBXO44 in the breast cancer cell line MDA-MB-231 was higher than that of the normal breast ductal epithelial cell line HBL-100,verifying the reliability of the results of immunohistochemistry and explaining FBXO44 may promote the development of breast cancer again.