Effect Of Fucoxanthin On Growth And Apoptosis Of HSC-T6 Cells And Its Mechanism

AIM: To explore the effect of fucoxanthin(FU) on the growth and apoptosis of HSC-T6 cells and its mechanism.METHAS: HSC-T6 cells were divided into blank control group, negative control group and drug group(treated with with different concentrations of FU). The cell activity was detected by CCK-8 assay at 24 h, 48 h and 72 h after FU treatment. The cell cycle distribution and apoptotic rate were analyzed by flow cytometry. The protein expression of Bcl-2 and Bax were detected by Western blotting.RESULTS: Compare with blank control group, the cell activity of HSC-T6 cells was inhibited by FU at concentrations of 15~75 ?mol/L with a dose-time-dependent manner(P<0.01). The cell ratio of G1 phase was significantly decreased(P<0.01) and the cell ratio of S phase and G2 phase was significantly increased(P<0.01) in 60 ?mol/L FU groups after 24 h. The cell ratio of G1 phase was significantly decreased(P<0.05) and the cell ratio of S phase and G2 phase was significantly increased(P<0.05) in 15 ?mol/L and 30 ?mol/L FU groups with a dose-dependent manner after 48 h. The early cell apoptotic rates and total cell apoptotic rates were significantly increased in the 3 FU groups with a dose-dependent manner(P<0.05). The protein expression of Bax was significantly increased in 3 FU groups and the protein expression of Bcl-2 was significantly decreased in 30 ?mol/L and 60 ?mol/L FU groups(P<0.05).CONCLUSIONS: FU inhibits the growth of HSC-T6 cells possiblely via arresting the cell cycle at S phase and G2 phase. The apoptosis of HSC-T6 cells induced by FU might be via down-regulating the protein expression of Bcl-2 and up-regulating the protein expression of Bax.