Bone Marrow Mesenchymal Stem Cell Transplantation Between Joint Pirfenidone Research For The Treatment Of Pulmonary Fibrosis

Objective:In pure dexamethasone, pure between bone marrow mesenchymal stem cells (MSCs) transplantation and pure Pirfenidone treatment of pulmonary fibrosis in rats as reference, on the basis of discussion Pirfenidone(PFD) joint between bone marrow mesenchymal stem cells (MSCs) transplantation in the treatment of bleomycin induced pulmonary fibrosis in rats.Methods:SD rats were isolated and cultured in vitro between bone marrow mesenchymal stem cells, to the fourth generation used in the experiment; 54 SD rats were randomly divided into negative control group (group A) and positive control group (group B), pure dexamethasone treatment group (group C), pure MSCs transplantation in treatment group (group D), pure Pirfenidone treatment group (group E) and Pirfenidone with MSCs transplantation in treatment group (group F), each 10 rats in each group. B to group F respectively by the trachea injection of bleomycin preparation of pulmonary fibrosis model. As the positive control group, not other processing after injection of bleomycin; Owed to group C injections for three consecutive days on day 2 after intraperitoneal injection of dexamethasone, group D should come immediately after injections by tail vein injection of rat bone marrow mesenchymal stem cell suspension; On the second day after the injection of bleomycin group E seven consecutive days to give; Group F should come immediately after injections by tail vein injection of rat bone marrowPirfenidone mesenchymal stem cell suspension, then the next day began for seven consecutive days to give;Pirfenidone As A model of group A negative control, the trachea injection amount of normal saline after 2 ml/kg, not for other processing. Building 7, 14,21 days after the batch to be put to death animals, to return the lung tissue and HE staining to observe morphological changes of lung tissue, and at the same time the immune histochemical method determination of lung tissue growth factor beta 1 (TGF-beta 1) expression.Results:Lai is made after the mould, C to F group rat inflammatory cells infiltration than group B decreased, significantly reduce pulmonary fibrosis degree and the pathological changes of the F the CDE group is lighter, pulmonary fibrosis is not obvious. Lung coefficient decreased significantly in group B, the difference was statistically significant compared with group A (P< 0.05). B to group F pulmonary fibrosis score higher than group A (P< 0.05); Group F score, the difference was statistically significant (P< 0.05). Transforming growth factor in lung tissue collagen expression on the number of positive cells decreased significantly, F the BCDE group decreased and the strength is more obvious, the group A compared with group B to F, the difference was statistically significant (P< 0.05), there was no statistically significant difference between the CDE group (P> 0.05).Conclusions:Bone marrow mesenchymal stem cell transplantation between joint Pirfenidone has certain inhibitory effect on pulmonary fibrosis, the expression of growth factors can reduce the lung tissue, have repair and regeneration effect, obvious effects of treatment for pulmonary fibrosis.