The Experimental Research On Ai Tong Xiao Formula Intervention In The T-lymphocyte Cells And Its Subsets Of Hepatocellular Carcinoma In Rats

Objective:To discuss the effects of Ai Tong Xiao formula on the T-lymphocyte cells and its subsets of hepatocellular carcinoma in rats,by observing the changes of T-lymphocyte cells and its subgroups in cell-mediated immunity of hepatocellular carcinoma in rats.Method: In this research,we adopted Walker-256 cancer cell line to establish 30 transplantated hepatocellular carcinoma models in male SD rats by direct injection into liver.At the same time we raised 10 normal SD rats,to contrast with the model groups at the same period.On the process of building transplantated hepatocellular carcinoma models two rats were died.On the seventh day after establishing transplantated hepatic cancer models,we chose 8 rats randomly,executed them and take their liver tissues,observed the pathological changes,confirm the success of establishing models.Then the 20 transplantated hepatic cancer model rats were randomly divided into two groups: the group of Ai Tong Xiao formula and negative control group,each group had 10 rats.On the eighth day after establishing transplantated hepatic cancer models,the rats in group of Ai Tong Xiao formula had accepted intragastric administration of Ai Tong Xiao formula(according to the equivalent dose of human as 63mg·kg^-1·d^-1 in10ml·kg^-1·d^-1),the rats in negative control group had accepted intragastric normal saline in equal quantity,Once per day,14 days after drug withdrawal.On the fifteenth day after intervention,all the rats of three groups extracting blood in the abdominal aorta,and then remove their hepatic tumors tissue.Complete stripping tumor tissue,measuring tumor diameter,weight and record.Using ELISE to detect the expression of AFP,and testing the number of the peripheral blood CD3⁺CD4⁺T cells and CD3⁺CD8⁺T cells and the ratio of CD3⁺CD4⁺/ CD3⁺CD8⁺by flow cytometry.Results: 1.Tong Xiao Fang group the rat's liver tumor diameter and the weight both smaller than those in the negative control group,the difference was statistically significant?P <0.05?.Compare with the normal control group,the negative control group's AFP expression level increased obviously;Ai Tong Xiao group compared with negative control group AFP expression level decreased obviously,the difference was statistically significant.From this experiment,we can conclude that Ai Tong Xiao formula has the inhibitory effect on the liver cancer cells.2.There are significant differences between the normal control group and negative control group,negative control group's CD3⁺CD4⁺Tcells and the ratio of CD3⁺CD4⁺/CD3⁺CD8⁺Tcells was significantly lower than the normal control group?P <0.05,?,and the CD3⁺CD8⁺T cells is greater than normal control group?P <0.05?,with statistical significance.Show that hepatocellular carcinoma in rats exists the phenomenon of a low T-lymphocyte cell-mediated immunity function and immune suppressive conditions.Compared with the negative control group,Tong Xiao Fang groupCD3⁺CD4⁺T cells and the ratio of CD3⁺CD4⁺/ CD3⁺CD8⁺T increased,while CD3⁺CD8⁺T cells decreased,point out that Ai Tong Xiao formula may accelerate elimination of hepatocellular carcinoma in rats CD3⁺CD4⁺T while suppressing the expression of CD3⁺CD8⁺T expression.That also show that Ai Tong Xiao formula can improve hepatocellular carcinoma in rats' T-lymphocyte cells immune suppressed state by increasing the cellular immune function.Conclusion:1.Tong Xiao Fang formula has the inhibitory effect on rat's Transplanted liver cancer.2.CD3⁺CD4 ⁺T cells and the ratio of CD3⁺CD4 ⁺/CD3⁺CD8 ⁺in hepatocellular carcinoma in rats' peripheral blood decreased,and CD3⁺CD8⁺T cells increased,indicating that hepatocellular carcinoma in rats exists the phenomenon of a low T-lymphocyte cell-mediated immunity function and immune suppressive conditions.3.Ai Tong Xiao formula can improve hepatocellular carcinoma in rats' T-lymphocyte cells immune suppressed state by increasing the cellular immune function.