Expression And Clinical Significance Of MiR-106a And MiR-10a In Endometrial Carcinoma

Objective: To detect the expression of miR-106 a and miR-10 a in endometrial carcinoma,atypical hyperplasia and normal endometrium tissues.And to investigate the relationship between the expression of miR-106 a,mi R-10 a and the origination,development,invasion and metastasis of endometrial carcinoma.Methods: The endometrium tissue samples from forty patients with endometrial carcinoma,twenty patients with atypical hyperplasia and thirty patients with normal endometrium(from the women with abnormal uterine bleeding and myoma uteri)were collected,poly(A)-RT-qPCR(SYBR Green)was employed to test the expression of miR-106 a and miR-10 a in different endometrial tissues.The statistical analyses were performed between the expression of miR-106 a,miR-10 a and the clinicopathological characteristics of endometrial carcinoma.Results:The relative expression of miR-106 a in endometrial carcinoma,atypical hyperplasia and normal endometrium tissues were 0.667(0.197,1.624),0.245(0.064,0.759)and 0.104(0.003,1.350),the expression gradually declined,and the difference was statistically significant(P<0.01).The expression of mi R-106 a in endometrial carcinoma tissues was higher than that in normal endometrium tissues(P<0.01),and atypical hyperplasia tissues(P<0.05),but the difference between normal endometrium and atypical hyperplasia tissues was no statistical significance(P>0.05).The expression of miR-106 a in stage ?+?of endometrial carcinoma patients was higher than stage?+?,in patients with lymph node metastasis was higher than that without lymph node metastasis,the difference was statistically significant(P<0.01);The expression of miR-106 a in different age,pathological type,pathological grades,myometrial invasion of endometrial carcinoma patients had no statistical difference(P>0.05);The expression of miR-106 a in endometrial carcinoma patients with ER positive or negative had no statistical difference(P>0.05);in endometrial carcinoma patients with PR positive or negative,the difference of expression had no statistical significance(P>0.05).The relative expression of mi R-10 a in endometrial carcinoma,atypical hyperplasia and normal endometrium tissues were 1.024(0.261,2.309),0.307(0.064,1.008)and 0.289(0.039,0.756),the expression gradually declined,and the difference in the three groups was statistically significant(P<0.01).The expression of miR-10 a in endometrial carcinoma tissues was higher than that in normal endometrium tissues(P < 0.01),and atypical hyperplasia tissues(P < 0.05),but the difference between normal endometrium and atypical hyperplasia tissues was no statistical significance(P > 0.05).The expression of miR-10 a in stage ? + ? of endometrial carcinoma patients was higher than stage?+?,in patients with grade 3 of pathology was higher than that 1+2,in patients that myometrial invasion ? 1/2 was higher than that < 1/2,in patients with lymph node metastasis was higher than that without lymph node metastasis,and the difference was statistically significant(P<0.05);The expression of miR-10 a in different age,pathological type of endometrial carcinoma patients and whether ER-positive,PR-positive had no statistical difference(P>0.05).Conclusio:The expression of miR-106 a was up-regulated in endometrial carcinoma tissues,and in stage ?+?of endometrial carcinoma patients was higher than stage?+?,in patients with lymph node metastasis was higher than that without lymph node metastasis,which indicated that miR-106 a may regulate the origination and development of endometrial carcinoma as an oncogene,and promote the invasion and metastasis of endometrial carcinoma.The expression of mi R-10 a was up-regulated in endometrial carcinoma tissues,and in patients with grade 3 of pathology was higher than that 1+2,it showed that miR-10 a was associated with the malignant degree of endometrial carcinoma and played a crucial role as an oncogene in the development of endometrial carcinoma.The expression of miR-10 a in stage ? + ? of endometrial carcinoma patients was higher than stage?+?,in patients that myometrial invasion?1/2 was higher than that<1/2,it was presumed that high expression of miR-10 a might promote the invasion and migration of endometrial carcinoma,by regulating the expression of related target genes to inhibit the synthesis and the expression of function of target protein.The expression of mi R-10 a in endometrial carcinoma patients with lymph node metastasis was higher than that without lymph node metastasis,which indicated that miR-10 a might promote lymph node metastasis of endometrial carcinoma.In summary,the expression of miR-106 a and miR-10 a in endometrial carcinoma tissues were increased,they might participate in the origination and development,promote the invasion and metastasis of endometrial carcinoma,though the regulation of target genes.So we demonstrated that mi R-106 a and miR-10 a were both oncogenes of endometrial carcinoma.