The Effect Of Human Cytomegalovirus(HCMV) On T Lymphocyte Subsets And NK Cells In Children

Objective:Human cytomegalovirus(HCMV)belongs to ? herpes virus,the genome of which can encode 250 proteins,the present studies have confirmed that at least 50 proteins are closely related to its biological characteristics,and a set of more mature infection-latencyreactivation mechanism was formed.So pathogenesis and immune mechanisms are different from other viral infections after the infection of HCMV to children,the primary infection and reactivation of HCMV may affect the immune function of the infected children.The process of HCMV causing disease is the result of interaction between HCM V and immune system.Lymphocyte is the most important part of body's i mmune system,when the number and function of different lymphocyte s ubsets and NK cells were abnormal,the immune function of body is diso rdered and will lead to an increased susceptibility to microorganisms.T lymphocyte subsets mainly are CD3+CD4+T and CD3+CD8+T lymphocytes,when the virus invades the body,they interact,often show impaired immune T lymphocyte subsets.NK cells are innate immune cells,an important class of effector cells against viral infections.T lymphocyte subsets and NK cells are known to play an important role in the body resistance to HCMV infection,children are more vulnerable to HCMV infection because of significantly lower T lymphocyte subsets and NK cells than normal adults.At present,the research only stays in the epidemiological investigation of HCMV susceptible population or the diagnosis of HCMV by HCMV-DNA virus detection,immune function and changes in the level of immune cells after infection in infected children were not further analyzed.In this project,by selecting the physical activity of children and HCMV infection in children as research subjects observed in HCMV infection in early childhood physical examination by serum antibody detection instruments,and analyze changes of T lymphocyte subsets and NK cell levels in the control group?latent infection group?re-infection group and active infection group.To provide a new theoretical basis for the study of immune mechanism and clinical diagnosis and treatment of children with HCMV infection,and finally reach the goal of ensuring the healthy growth of children.Methods:261 cases of children(2.3 to 3.0 years old,average age(2.6±0.3)years old)in a kindergarten entrance examination in 2015 were chosen.All the selected children were detected by HCMV-IgG,HCMV-IgM,infection of HCMV in children before admission was observed.Grouping according to the test results:Set the HCMV-IgG(-)HCMV-IgM(-)group as the control group;Set the HCMV-IgG(+)HCMV-IgM(-)group as the latency infection group;Set the HCMV-IgG(+)HCMV-IgM(+)group as the re-infection group;Set the HCMV-IgG(-)HCMV-IgM(+)group as the primary infection group.In addition,101 cases of hospitalized children with HCMV-IgG(+),HCMV-IgM(+)and urine HCMV-DNA(+)were chosen as the active stage infection group,from January 2015 to December 2015,admitted to Department of Pediatrics in Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital because of HCMV infection.aged 2.4 to 3.1 years,average age(2.7 ± 0.4)years old.T lymphocyte subsets(CD3+T,CD3+CD4+T,CD3+CD8+T)and NK cell(CD3-CD16+CD56+)levels were detected in each group,changes of immune indexes in HCMV patients in different infection stages were observed.Results:1 HCMV infection in 261 childrenIn 261 cases of children,89 cases of children(accounting for 34.10%)were diagnosed as the latent period of infection(HCMV-IgG positive and HCMV-IgM negative);70 cases of children(accounting for 26.82%)were diagnosed as the re-infection period(HCMV-IgG positive and HCMV-IgM positive);102 cases of children(accounting for 39.08%)were diagnosed as the no infected children(the control group);0 cases of children was diagnosed as the primary infection(HCMV-IgG negative and HCMV-IgM positive),it was not detected.2 The levels of T lymphocyte subsets and NK cells in children in the different groupsCompared of the number of CD3+T lymphocytes: The active infection group were significantly decreased compared with the other groups,the difference was statistically significant(P<0.05).Compared of the number of CD3+CD4+T lymphocytes: The active infection group were significantly decreased compared with the other groups,the difference was statistically significant(P<0.05).Compared of the number of CD3+CD8+T lymphocytes: The active infection group were significantly increased compared with the other groups,the difference was statistically significant(P<0.05).Compared of the number of the NK cells(CD3-CD16+CD56+): The active infection group were significantly decreased compared with the other groups,the difference was statistically significant(P<0.05).the latent infection group and the re-infection group were significantly decreased compared with the control group,the difference was statistically significant(P<0.05).Conclusions:1 HCMV infection rate is higher in children before the nursery,mainly in latent infection period.2 When in the inactive infection period,the number of NK cells are only impactde by HCMV.3 When in the active infection period,the number of T lymphocyte subsets and NK cells were all impactde by HCMV.suggesting that cellular immunity in children with HCMV was changed.