Experimental Study Of Atlantoaxial Structural Disorder And The Entire Complex Effects On Blood Pressure

Objective: Optimization of cervical pre-hypertensive model; Observed Changes in rats after correction atlantoaxial joint structural disorder of blood pressure; To investigate the role of the vagus nerve in the cervical hypertension model.Methods:1. Select 130 SD male rats weighing(250±10g), divided into 30 groups, sham30, and the remaining 70 for copying atlantoaxial rats disorder, according to the inclusion criteria selected 30 as the left side of the surgery group, 30 as the right of the surgery group.2. United States respectively IItc Life science noninvasive blood pressure measurement system and multi-channel polygraph POWERLAB same time gr asping the left and right side of the surgery group, the control group and the sham blood pressure and heart rate. The first 7-day surgery(model successfu lly copied), the randomly selected half of Ach group of rats, measured using the BL420 system cervical vagus nerve action potential, and abdominal aortic blood, ELISA assay Ach content.3. The remaining left and right side of the surgery group underwent the second surgery, removal atlantoaxial joint space fixture, lifting joint disorders, postoperative blood pressure measurement, and the sham control group of normal feeding, the equivalent operation group gathering time blood pressure was observed in rats changes in blood pressure. Spass17.0 data using statistical analysis to compare e Ach group blood pressure, heart rate and the vagus nerve discharge, Ach index changes.Results:1. The left side 3 days after surgery group survival rate was 96%, 89% success rate model. The right of the surgery group survival rate was 93%, 84% success rate model. The left side of the surgery group compared with the previous experimental groups(P <0.01), there was a significant difference in the survival rate and improvethe success rate of model group was significantly better than the pre-test e Ach model group.2. The first surgery(implant fixture), the first 1,2,3,5,7 days left and the right side of the surgery group SBP, DBP were significantly higher than those in sham group(P <0.01), the left operation group SBP, DBP than the right operation group tended to increase significantly(P <0.05); no significant difference(sham group and control group SBP, DBP> 0.05);After the second operation(remove the fixture to relieve joint disorders), the first day of the left and right operation group SBP, DBP compared with sham group increased(P <0.05), the first 2,3,5,7 days left right surgical group SBP, DBP and sham no significant difference(P > 0.05); the right of the surgery group SBP, DBP decreased significantly compared with the left side(P <0.05); the control group and the sham group showed no significant difference(P > 0.05).Left and right the first time after surgery group SBP, DBP compared with the preoperative(P <0.01), blood pressure increased significantly; the second after the first postoperative SBP, DBP have more decreased(P < 0.05); before and after the sham group and control group two operations SBP, DBP compared(P > 0.05), no significant difference.3. The rats heart rate higher than 300 BMP, model group were higher than sham group(P <0.05); the right of the left side of the surgery group than in the surgery group(P <0.05); sham group and control group without significant difference(P >0.05). HRV in LF / HF index model group increased compared with sham(P <0.05);the right of the left side of the surgery group compared with the surgery group(P >0.05), LF / HF group is higher than the right side of the operation; sham group compared with the control group(P > 0.05), no significant difference.4. model e Ach group vagus nerve discharge frequency were lower than the sham group(P <0.05); the right of the left side of the surgery group compared with the surgery group(P <0.05), higher discharge frequency operation group on the right;and sham no significant difference compared to the control group.5. Compared with the sham-operated significantly decreased(P <0.05) content in rat serum Ach 5. The model group; the left and right side of the surgery group compared to serum Ach content(P <0.05), higher than the right side of the left; sham group and control group compared to(P > 0.05), no significant difference;6. Ach group BP and HRV index LF / HF, serum Ach content, vagus nerve discharge frequency of negative correlation.Conclusion:1. Optimized cervical hypertension model group from the previous month for e Ach experimental model consisting of high power, high blood pressure and the trend is clearly2. After releasing atlantoaxial joint rats, blood pressure return to normal.3. Rats atlantoaxial disorders can cause high blood pressure, can affect the vagus nerve, causing the firing rate slows down, heart rate and heart rate variability changes in serum Ach decreased.4. Ach group BP and HRV index LF / HF, serum Ach content, vagus nerve discharge frequency of negative correlation.