An Exploratory Study Of The Association Between The GCKR Gene Rs780094 Polymorphism And NAFLD In Han Population In Qingdao Area

Background and Objective: Nonalcoholic fatty liver disease(NAFLD)is one of the most prevalent causes of chronic liver disease worldwide.Advanced stages of which could lead to liver fibrosis,cirrhosis,and hepatocarcinoma.Therefore,it is important to study the pathogenic factors of NAFLD in the prevention and treatment of the disease.The pathogenesis of NAFLD is not completely clear at present.NAFLD is a genetic-environmentmetabolic-stress-related disea,genetic factors play an important role in the pathogenesis of NAFLD.Glucokinase regulatory protein(GCKR)polymorphisms is associated with type 2 diabetes mellitus,obesity,high triglyceride,gout,chronic kidney disease and other diseases.However,there was a controversy results about the relationship between GCKR gene polymorphism and NAFLD in Chinese Han population,the main purpose of this study is to investigate the association between GCKR gene rs780094 polymorphisms and NAFLD in a Qingdao Han population.Methods: This case-control study enrolled 160 patients(83 men,77women)with color Doppler ultrasound confirmed NAFLD and 172 control subjects without NAFLD(90 men,82women)from October 2013 to September 2013.All subjects were recruited from Qingdao Municipal Hospital.We test the following data:sex,age,height,weight,waist circumference?hip circumference?serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),gamma-glutamyl transpeptidase(GGT),low densitylipoprotein(LDL)and serum triglyceride(TG)level.We defined Body mass index(BMI)as weight/height2(kg/m2).The clinical and laboratory values were expressed as means ± S.D.As different data,Pearson Chi square test,logistic regression analysis,t test were used in this study.All the subjects were genotyped for rs780094 by polymerase chain reaction(PCR)and DNA sequencing method.Genotype and allele frequencies were compared between the contrl and case groups.Relationships between rs780094 polymorphism and NAFLD were tested.Statistical analysis was performed using SPSS statistical software,version 17.0 for Windows.Results: There was no significant association between sex(P>0.05).the NAFLD subjects show significant differences in several clinical and laboratory characteristics in this study,such as waist circumference?hip circumference?ALT?AST?GGT?LDL and TG level compared with the controls(P <0.05).The frequency of rs780094 T allele was similar to 59.1% in NAFLD patients and 57.6% in control group(P>0.05).the variant GCKR rs780094 T allele had not increased the risk of NAFLD in the Han population in Qingdao area compared with the C allele(OR= 1.064,95% CI: 0.781~1.449,P= 0.694),After adjustment for confounding factors,Logistic regression model analysis showed that compared with CC homozygous carriers,carrying the allele TT of T + CT genotype had not increased the risk of NAFLD.Conclusion: The GCKR rs780094 T allele was not significantly associated with increased risk of NAFLD in Qingdao Han population.