Frequency Changes Of Peripheral Blood Dentritic Cells And The Relevance With Clinical Symptoms In Patients With Parkinson's Disease

Background: Parkinson's disease(PD)is one of the most common neurodegenerative diseases in elderly people,it's main pathological characters are a progressive degeneration of midbrain dopaminergic neurons of the substantia nigra and the presence of Lewy bodies.In recent years,the idea neuroinflammation plays an important role in the pathogenesis of Parkinson's Disease(PD)has been paid more and more recognition,although its underlying mechanisms are still not well defined.Dendritic cells(DCs)is known as the most powerful professional antigen-presenting cells(APC),and play an important role in initiating and regulating innate and adaptive immune responses.As an important immune cell,we hypothesis DCs may be involved in the neuroinflammation mechanism of PD,and contribute to the initiation and progress of the disease.Objective: 1.To investigate whether the frequency of DCs changes in the peripheral blood in patients with Parkinson's Disease(PD).2.Find out the relevance between the frequency changes of DCs and the clinical assessments of PD(such as H-Y stage,the severity of motor and non-motor symptoms and so on).Method: The study collected 50 cases patients with idiopathic Parkinson's disease of the Neurology department of the First Affiliated Hospital of Dalian Medical University from Aug 2014-May 2015(male=26,female=24),the control group include 24 age-gender matched healthy individuals at the same period(male=8,female=16).The H-Y stage and anti-parkinson medication use evaluation was assessed by one same neurologist who also evaluated the UPDRS III scores,non-motor symptoms scale of the PD patients.The cognitive and emotional situation was assessed using MMSE,MOCA,HAMA and HAMD scales by one same neuropsychological practitioner.After collecting the fasting venous blood samples in the morning,the frequency of double labelled antibody positive DCs was detected by flow cytometry analysis,and calculated the percentage of each double positive DCs subset.Comparison between patient and control DCs measures and their relationships with clinical assessments were evaluated.Results: 1.The Lymphocytes DCs include LCD45RA~+CD45~+ ? LCD1c~+CD45~+ ?LCD14~+CD45~+DCs and the Monocytes DCs like MCD209~+CD45~+?MCD11b~+CD45~+?MCD1c~+CD45~+DCs were higher than those in the control group;while the percentage of LCD209~+CD45~+?LCD11b~+CD45~+? LCD86~+CD45~+ DCs ?MCD45RA~+CD45~+?MCD86~+CD45~+ ?MCD14~+CD45~+DCs declined compared with control group,but there were no significant difference(p>0.05).2.About the same age stages: The MCD1c~+CD45~+DCs of patients in the “?60 group” significantly increased compared with the healthy people(*p=0.041);Only the CD14~+CD45~+DCs of the PD group has a significant positive correlation with age(L,r=0.341,p=0.015;M,r=0.381,p=0.006),the left phenotypes have no significant relevance with age.While in the control group no significant relationships were observed between age and DCs subsets.3.The LCD86~+CD45~+DCs was negatively correlated with the duration of PD(r2=0.209,non standardized coefficient =-0.15,p=0.001),while the MCD209~+CD45~+DCs was positively correlated with the duration(r2=0.16,non standardized coefficient=0.153,p=0.006).4.In the PD group there was a significant negatively relationship between the LCD86~+CD45~+ DCs and H-Y staging(r2=0.065,non standardized coefficient =-0.36,p=0.028).As for the UPDRS III,no significant correlation was observed between percentage of each phenotype DCs subset and the UPDRS III scores.5.The MCD45RA~+CD45~+ DCS was positively correlated with the non-motor symptoms scales(r2=0.18,non standardized coefficient =1.325,p= 0.027)and the MMSE scales(r2=0.016,non standardized coefficient=1.039,p= 0.031).While the MCD14~+CD45~+DCS has a negative correlation between MMSE(r2=0.216,non standardized coefficient =-0.255,p=0.001)and MOCA scales(r2=0.299,non standardized coefficient=-0.183,p=0.002).About the emotional tissues,there were no significant correlations between each phenotype DCs subset and both HAMA and HAMD scales(p>0.05).6.No significant relationships were found in PD patients between daily anti-parkinson medication use and the percentages of each phenotype DCs.The MCD14~+CD45~+DCs and gender showed a significant negative correlation(r=-0.322,p=0.031*),which means the MCD14~+CD45~+ DCs in male patients was higher than female,while other phenotypic DCs percentage and gender had no significant relationships.Conclusion: 1.The frequency of CD14~+CD45~+ DCs in the peripheral blood of PD patients increased along with growing age.2.Some DCs subtypes levels in the peripheral blood of PD patients decrease or increase as PD specific symptoms(motor and cognitive dysfunction)become more severe,suggesting that these cells likely represent a biological index of PD progression and treatment situation.