The Study Of Preliminary Mechanism In Human Gliomas With TWIST2 Gene

Objective:1?To examine the expression level of TWIST2 in human Gliomas;2?To investigate whether the grade malignancy of Gliomas is associated with TWIST2,and discuss the possible mechanisms. Methods:1?To collect 60 cases Glimas and 20 cases nontumorus brain tissue which belonged to the Department of Neurological Surgery affiliated to the first hospital of Nan C hang University from 2010 to 2015. And the clinical Pathology information and formalin- fixed Paraffin-embedded were asked for integrity. According to the World Health Organization(WHO) standard and demand of expriment, all of the case will be divide into four groups. Every group has 20 cases.The first group: Nontumorus; The two group: WHOII;The third group: WHOIII;The fouth group: WHOIV. Use Immunohistochemistry(IHC) and Western Blotting to detect the expression level of TWIST2 Protein in each team.2?To gather 61 cases surgery fresh Glimas tissue and 12 cases nontumorus brain tissue which belonged to the Department of Neurological Surgery affiliated to the first hospital of Nan C hang University from 2014 to 2015(which had been certified by Pathology). Use qRT-PCR and Western blot to detect the level of TWIST2 mRNA and Protein which originate from fresh Glimas tissue respectively and app ly Western blot to test the level of Protein of E-cadherin?N-cadherin?Vimentin which originated from colletcted fresh Glimas tissue respectively. Results:1?IHC was applied to analysed the level expression of TWIST2 in human Gliomas of different Grade,The results show TWIST2 is expressed in human Gliomas and the level of TWIST2 Protein was different.Using qRT-PCR and Western blot to detect the expression level of TWIST2 mRNA and Protein in fresh Gliomas tissue,The results show the expression level of E-cadherin?N-cadherin?Vimentin Protein is negative/ positive correltion to the level of TWIST2 Protein. Conclusions:1?TWIST2 is expressed in human Gliomas,and the expression level of is Positive correlation to the human Gliomas Grade.2?TWIST2 gene may be influent the malignance of Glimas by EMT.