| As essential fatty acids, polyunsaturated fatty acids(PUFAs) are of great significance to the physiological function andhealth of human. Among them, docosahexaenoic acid(DHA) and eicosapentaenoic acid(EPA) of ?-3 PUFAs have high nutritional values and have been widely applied as nutritional supplements. In addition to fish oil, DHA can also be isolated from microalgae.In this study, we studied the oil production and accumulation in Crypthecodinium cohnii ATCC 30772. Avariety of chemical inducer compounds(i.e., butylated hydroxyanisole, propyl gallate, biotin, sodium malate, lovastatin) was added exogenously into the cultivation andused to induce the algal cells' oil accumulation. The induction experiments showed that, except propyl gallate, all other inducers can increase the total fatty acid content of C. cohnii, among which butylated hydroxyanisole increased lipid content by 8.8%, biotin increased by 2.8%, sodium malate increased by 6.43%, lovastatin increased by 4.16%, respectively, when compared with those of the control groups. To explore the molecular mechanisms responsible for the increased fatty acid contents, we employed gas chromatography-mass spectrometry(GC-MS) and liquid chromatography-mass spectrometry(LC-MS) to conduct metabonomics analysisof butylated hydroxyanisoletreated samples, which had the greatest increase in lipid content. Through the principal component analysis(PCA) of the metabolomic data, we identified metabolites potentially related to increased oil accumulation, such as ADP, NADPH, R5 P and PEP. Through a weighted gene coexpression network analysis(WGCNA), we further determined that 8 models and 9 hub metabolites potentially related to the BHA treatment. These results revealed possible regulation mechanisms related to the increase of the fatty acid accumulation. |
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