Study On The In Vitro Antibacterial Activity Of Compound Zaofan Functional Shampoo On Malassezia

bjective: Malassezia is seborrheic dermatitis on common bacteria.In this research,both agar diffusion method(punch method)and microdilution method in vitro drug sensitivity test method are used to observe the in vitro antibacterial activity of compound zaofan functional shampoo drug on Malassezia.Methods: 1.By agar diffusion method(punch method),compound Zaofan functional shampoo drug were diluted into concentration of 2g/ml,1 g/ml,0.5 g/ml,0.25 g/ml,0.125 g/ml.and Ketoconazole Lotion were diluted into concentration of 0.01 g/g? 0.005 g/g ?0.0025 g/g ?0.00125 g/g ? 0.000625 g/g.Prepare inoculant suspension without medicament for positive control group,and non inoculated suspension without medicament for negative control group.After the agar plate culture medium is inoculated with the activated Malassezia suspension,put it in the incubator with 35 degrees Celsius for 72 h.,then directly measurement of the inhibition zone diameter(mm)by vernier calipers.2.Liquid medium microdilution method,improved from Yeast micro dilution method in the M27-A program by the national clinical laboratory standardization committee(NCCLS),is used to observe the effect of Compound Zaofan functional Shampoo drug at different concentrationsin vitro inhibition of Malassezia,comparing to the Ketoconazole Lotion diluted into different concentrations,containing 4% olive oil as a drug sensitivity test of liquid medium.Establish negative control group without Malassezia and normal growth control group without medicament.And measure Compound Zaofan functional shampoo drug MIC value.Results: 1.agar diffusion method(punch method): 2 g / ml,1 g /ml,0.5 g / ml,0.25 g / ml,0.125 g / ml concentrations of compound Zaofan functional shampoo drug for bacteriostasis diameter of Malassezia are 59.6 mm ?48.4mm ?24.3mm ?15.4mm? 11.0 mm.0.02 g/ml?0.01 g/ml ?0.005 g/ml ?0.0025 g/ml ?0.00125 g/ml concentrations of Ketoconazole Lotion for bacteriostasis diameter of Malassezia are81.6mm ?69.9 mm ?47.2mm ?30.4mm ?19.9mm?2.microdilution method : Compound Zaofan functional shampoo drug solution MIC value is 0.005g/ml.Conclusion: Compound Zaofan functional shampoo drug has strong inhibitory effect in vitro of Malassezia.Inhibitory effect is related to drug concentration.there was no statistically significant difference effect between clinical strains and standard strains.Compound Zaofan functional shampoo drug solution MIC value is 0.005g/ml.