| Objective: Through the establishment of rabbit model of parietal critical bone defects in this study. By comparing and observing Relatively Composite membrane(platelet rich fibrin and collagen membrane) simple platelet rich fibrin membrane and collagen membrane(BME-10X) three membrane in the repair of rabbit cranial bone defect in different periods of guiding bone tissue regeneration ability. The characteristics and advantages of the application of the three kinds of membranes were analyzed, which provided the reference for the selection of barrier membrane in the oral implant surgery.Methods: 24 male Japanese big ear rabbits were established 3 bone defects in rabbit calvarial bone and divided into three groups. Near the left ear cranial suture composite membrane group, right is the collagen membrane group, away from the ears left for the PRF membrane group. Filling materials according to the defect area are as follows: Composite membrane group film(PRF film and collagen membrane), PRF group film(PRF), collagen membrane group film(collagen membrane). After 2, 4, 8 and 12 weeks, 6 animals were sacrificed at operation and the were observed morphological bone defect area of each group and degradation of membrane. The tissue repair and regeneration of bone defect area were observed by X ray, HE staining, Masson staining and histological observation.1.Morphological observation: In the early(after 2 weeks, 4 weeks) PRFresidual, etained in the later period(after 8 weeks, 12 weeks PRF) degradationcompletely; In the whole time observation node collagen membrane all or part,PRF group barrier function compared with collagen membrane, compositemembrane group of poor.2.X-ray observation: After 2 weeks, the PRF group, collagen membranegroup coloboma area edge sharp and bone density were low, the compositemembrane group edge see a little fuzzy, low bone density, After 4 and 8 weeksoperation, fuzzy edge of the three groups of the defect area, expand the scope ofthe high density, composite membrane group range greater than the PRF group,collagen membrane group; After 12 weeks, composite membrane group, thedefects were bone density and the surrounding normal bone density similar,PRF group, collagen membrane group, the bone density is normal to slightlylow.3.HE staining: New bone in the score, postoperative 2, 4, 8 and 12 weeks,composite membrane group compared with PRF membrane and collagenmembrane group were higher, significant difference(P < 0.05); 4 and 8 weeksafter operation, the PRF membrane group > collagen membrane group, thedifference was significant(P < 0.05); after 2 weeks and 12 weeks, PRFmembrane group and collagen membrane without statistically significantdifference(P > 0.05). 4. Masson staining: Analysis on the value of the average optical density of Result: collagen fiber: postoperative 2, 4, 8 and 12 weeks, the composite membrane group to promote the ability of bone maturation were better in PRF membrane group, PRF membrane group good on the collagen membrane group, namely composite membrane group > PRF membrane group > collagen membrane group, the difference was significant(P < 0.05).Conclusion:1.Compared with the PRF membrane, the degradation time of the collagen membrane in the composite membrane and the collagen membrane group was longer than that of the membrane, which played a more significant role in the barrier function.2.The early osteogenesis ability of PRF membrane group was better than that of collagen membrane group.3.The new bone mass of the composite membrane group was significantly higher than that of the PRF group and collagen membrane group.4. The ability of composite membrane group to promote bone maturation was better than that of PRF group and collagen membrane group. |
