| Objective: Based on the previous research of “bone controlled by gall bladder meridian” which verifies that gall bladder meridian acusectoring has “collection and adjustment” effect toward the bone rebuilding to research that the gall bladder meridian acusectoring whether has similar adjustment effect on bone metabolism and glycometabolism of type-II diabetic rat.Methods : Randomly choose 6 rats to normal control group from 66 SD male rats with 200±20g weight;after model construction,randomly choose 40 rats;then combining weight and blood glucose,through blocked randomization method to divide the 40 rats into 5 groups with 8 per group,namely acupoint group of 11 pm to 1am,acupoint group of 7am to 9am,non-acupoint group of 11 pm to 1am,non-acupoint group of 7am to 9am and modeling control group;the construction model rats,after fed by high-fat and high-sucrose fodder for 4 weeks,are injected 35mg/kg STZ solution on the enterocoelia;the normal group is fed by general fodder,and be injected 35mg/kg citric acid · sodium citrate buffer solution on the enterocoelia;Taking blood from caudal vein to measure random blood glucose of 3,7,10 and 14 days respectively after modeling and collect the rats of ≥16.7 mmol/L random blood glucose.In the third week after model construction,the groups of gall bladder meridian and groups of non-gall bladder meridian are conducted acusector according to its time.Intervene meridian point: Yanglingquan(GB34),Huantiao(GB30),Xuanzhong(GB39),non-meridian point: choose non-meridian point of the rat on interior part of its hind legs,both near and far points of the same side each time.The acupoint is chose on the basis of rotate of left and right side.The model control group and normal control group are free of intervention.During the first 5 intervention course,the feed way is same to the before of mode construction.From the 6th treatment course,all rats are fed with general fodder.After 5 weeks intervention,collecting 1-2ml heart blood from live rate to test biochemical criterion of osteocalcin and other items.After 10 weeks intervention,kill the rats.Before killing them,they were fasted for 12 h without depriving water and injected 2% Nembutal in their enterocoelia on the basis of 0.25ml/per 100 g of weight;After anesthesia,take 10 m L blood from their aorta abdominalis and then put serum to-80℃ freeze chest for reserve and further index text.Take pancreas to fix in 10% formalin solution under room temperature to conduct pathological section.Quickly take out the rat’s femur and tibia on one side,carefully remove muscle and connective tissue attachment and fix in 4% formalin solution under room temperature to measure bone density.Results: 1.During the two weeks of model construction,the model rats have the phenomenon of polyphagis,dieresis,weight lose and obviously hyperglycemia(random blood glucose ≥16.7mmol/L)etc which indicate the success of model construction.2.After acusector intervention of 5 weeks,the acupoint group of 11 pm to 1am,and acupoint group of 7am to 9am have higher blood glucose level than non-acupoint group of 11 pm to 1am,non-acupoint group of 7am to 9am and model group,and the decrease difference of acupoint group of 11 am to 1am has statistical significance(P<0.05),while the acupoint group of 11 am to 1am has the trend of blood glucose decrease but relatively higher to acupoint group of 7am to 9am with no statistic difference.After 10 weeks acusector intervention,the acupoint group of 11 pm to 1am has lower blood glucose than non-acupoint group of 11 pm to 1am,non-acupoint group of 7am to 9am and model group,and the difference has statistical significance(P<0.05),while the acupoint group of 7am to 9am has no blood glucose value difference with other groups.In addition,the acupoint group of 11 pm to 1am and non-acupoint group of 11 pm to 1am have instantly blood glucose decrease that after 120 min of acusectoring,the blood glucose decrease significantly(P<0.05);The two groups have no significant difference.3.Fasting serum insulin(FINS): compared with the model group,the content of FINS in the normal group and electro acupuncture intervention group was significantly lower,and the difference was statistically significant(P<0.01).Fins content in the non-acupoint group of 11 pm to 1am,non-acupoint group of 7am to 9am is higher than in the acupoint group of 11 pm to 1am and acupoint group of 7am to 9am,the differences between non-acupoint group of 11 pm to 1am and acupoint group of 11 pm to 1am,acupoint group of 7am to 9am were statistically significant(P < 0.05).The insulin sensitivity index(IAI): compared with the model group,IAI in normal group,acupoint group of 11 pm to 1am,acupoint group of 7am to 9am,and non-acupoint group of 7am to 9am are significantly higher,the difference is statistically significant(P < 0.01).Compared to the non-acupoint group of 11 pm to 1am,non-acupoint group of 7am to 9am,IAI in acupoint group of 11 pm to 1am,acupoint group of 7am to 9am and normal group was lower,the difference between the non-acupoint group of 11 pm to 1am and acupoint group of 11 pm to 1am,acupoint group of 7am to 9am and normal group has statistical significance(P < 0.05).Insulin resistance index(IRI): compared with the model group,IRI in normal group and electro acupuncture intervention is lower,and the difference between the normal control group,acupoint group of 11 pm to 1am and acupoint group of 7am to 9am and model group has a statistical significance(P < 0.05).4.The pathologic histology change of pancreas: Comparing to normal group,the endocrine portion number of diabetic rat decreased to some degree,among which the model group has the smallest number with progression necrosis of pancreas.Among intervention groups,the acupoint group of 11 pm to 1am and acupoint group of 7am to 9am have more endocrine portions and comparable completed cell structure of pancreas with partly nucleolysis phenomenon.And the morphological structure of acupoint group of 11 pm to 1am is more similar to normal group,the non-acupoint group of 11 pm to 1am and non-acupoint group of 7am to 9am have endocrine portion,and disordered orientation of pancreas cell with unclear karyotheca and necrosis phenomenon of karyopyknosis.5.BMD change: Comparing to normal group,the BMD of acupoint group of 11 pm to 1am and non-acupoint group of 11 pm to 1am have decrease(P<0.05)which has statistic significant;while the BMD of diabetic rats have little BMD difference with no statistic significant.6.After 5 weeks of acusector intervention,comparing to normal group,the bone glaprotein of diabetic rats are decreased significantly and the difference has importantly statistic significance(P<0.01),while the acusectoring intervention groups(excluding acupoint group of 7am to 9am)have higher osteocalcin than model group but without statistic significance.After 10 weeks of electro acupuncture intervention.Compared with the normal group,the serum osteocalcin content in acupoint group of 7am to 9am,non-acupoint group of 7am to 9am and model group decreased significantly,the difference was statistically significant(P < 0.05);and osteocalcin content in the acupoint group of 11 pm to 1am,non-acupoint group of 11 pm to 1am decreased,but there was no statistical significance.Osteocalcin content in acupoint group of 11 pm to 1am and non-acupoint group of 11 pm to 1am is higher than in acupoint group of 7am to 9am and non-acupoint group of 7am to 9am and they have statistical difference with non-acupoint group of 7am to 9am(P < 0.05).Conclusion: 1.Through acusector intervention,the blood glucose of treatment groups have decreased,among which the acupoint group of 11 pm to 1am decrease the most.The effect of acusector is obvious within 2 hours with little difference.2.Electro acupuncture for gall bladder meridian can improve insulin resistance and insulin sensitivity,increase the usage rate of insulin,but there were no significant differences among groups in different hours.3.The OMD changes of most type-II diabetic rats are small and the effect of acusector is not significantly three months after the modeling.4.The acusector intervention may has effect to osteocalcin,however,this needs further test by excluding peak value and unifying the time of taking samples. |
PDF Full Text Request