Study The Antihyperlipidemic Effect Of The Licorice Flavonids And Related Mechanism

Objective: To study the hyperlipidemia effect of the flavonoids extract parts(Licorice flavonoids, LF) from Glycyrrhiza uralensis in high-fat diet feeding rat model was employed to evaluate the effect by animal blood biochemical experiments and western blot were used to exploring the antihyperlipidemic mechanism in relationships associated with the PPAR?/AMPK signaling pathway.Method: 120 male Sprague-Dawley(SD) rats were randomly assigned into 2 groups as control, high-fat diet group. Control group were fed maintenance feedstuff and high-fat diet group were fed high-fat feedstuff for 90 consecutive days. During this time we monitoring weight and food intake of rats. Then high-fat diet group was assigned into 2 group as high fat feedstuff sensitive group and high fat feedstuff insensitive group according to weight of rats. High fat feedstuff sensitive group were randomly assigned into model group, fenofibrate group(40mg/kg, ig), metformin(300mg/kg, ig)and 3 LF groups( LF 50, 150, 400 mg/kg, ig).During this time we monitoring weight and food intake of rats,medicine was given by intregastric administration. At the end of the experiment, detect the weight and food intake, calculate fat index, determinate serum lipid parameter(TG,TC,LDL,HDL) and antioxidant index MDA and SOD to assess the antihyperlipidemic effect.The changes of the PPAR?, AMPK protein expression in liver were examined by western blot.Result:1.LF effect food intake experiment rats: Food intake of each group rats no significant difference(P>0.05).2.LF effect weight gain rate of experiment rats:Compared with the model group,Flavonoids can reduce weight gain rate(P<0.05 or P<0.01).3.LF effect fat index of experiment rats:Compared with the model group,Flavonoids can reduce fat index(P<0.05 or P<0.01).4.LF effect serum lipid parameter of experiment rats:Compared with the model group,Flavonoids can reduce TG,TC,LDL(P<0.05 or P<0.01)and promote HDL in serum(P<0.05 or P<0.01).5.LF effect Antioxidant index of experiment rats:1)MDA:Compared with the model group,Flavonoids can reduce MDA(P<0.05 or P<0.01).2)SOD:SOD of each group rats no significant difference(P>0.05).6. Western blot results:1)Compared with the normal group,PPAR? protein expression decreased in liver of model group(P<0.05).Each drug group can improve the level of PPAR? protein expression in liver(P <0.01).2)Compared with the normal group,AMPK protein expression decreased in liver of model group(P<0.05). Each drug group can improve the level of AMPK protein expression in liver(P <0.01).3)Compared with the normal group,p-AMPK protein expression decreased in liver of model group(P<0.05).Each drug group can improve the level of p-AMPK protein expression in liver(P <0.05 or P <0.01).Conclusion: The flavonoids extracted parts from Glycyrrhiza uralensis may produce the hyperlipidemia effect in high-fat diet feeding model in rat, and its relevant mechanisms may be associated with the increasing PPAR? protein expression to upregulate AMPK and p-AMPK protein expression and attenuate hyperlipidemia.