Design And Implementation Of Three-channel Line Scanning Fluorescence Imaging

Now it is necessary for people to figure out the brain's operating mode for the demand for research and development of artificial intelligence, as well as brain disease treatment. Many tools exist to study the human brain, but only optical microscopy imaging techniques can be used to study the various connections and interactions between neurons in large-volume nervous tissue with the resolution of submicron and then the operating mode of neural network can be more profoundly understood. Usually people use mouse brains as a model to study the brain's neuronal connections and operating modes.The fluorescent protein marker is a kind of important neural tracer. Division of Biomedical Photonics in Wuhan National Laboratory for Optoelectronics(CBMP) developed a fluorescence imaging system based on line scanning and chemical tomography(TDI-fMOST), which uses stage-scan imaging mode. This system is more stable than other systems. But to transform it into a three-color imaging system, there are still many problems to solve.This paper analysed the optical and mechanical design requirements for the the three channel imaging expansion according to the characteristics of fast line scan fluorescence imaging system and a three channel imaging module that can connect three TDI-CCDs to image simultaneously was designed and realized. The author also analyzed the multi-channel imaging optical module and the special requirements of surface roughness. The experimental verification was done after that. The imaging quality is ensured through the selection and the design of fixed structure of filters. At last the author carried on the test for the three channel imaging module, and applied it to the imaging system. The usability of the system is verified by using standard samples and biological samples.