Stilbene Huang Bao Renal Party Affect Renal Failure Were Pax2 Expression

Objective:Renal failure on the observation of stilbene Huang Bao renal party baby mice kidney tissue matching box gene 2(Paired box2, PAX2) expression and the effect of explore stilbene Huang Bao renal party protection of renal function, delay the action mechanism of renal failure in children.Experimental subject:Weight 80-120 g around age 65 SD baby mice, were bought in second affiliated hospital of Harbin medical university animal center. During the experiment, all were raised in the center of the heilongjiang university of Chinese medicine animal experiments. Room temperature at 22 to24 ?, relative humidity(50-70) %, artificial lighting 12 h day/night cycle. Keep ventilation, quiet,regular replacement bedding, disinfection of laboratory animal cages. Animals free water, normal diet. All food is provided by heilongjiang university of Chinese medicine animal experiment center and passes through the strict disinfection.Experimental method:According to the random grouping method to the 65 were random points in the five groups:control group, model group, Chinese caterpillar fungus, stilbene Huang Bao renal party group(hereinafter referred to as yellow stilbene group), stilbene Huang Bao renal party+ cp group(that is, the joint group). Kept separately in each group, made a mouse pup model kidney failure,unilateral ureteral ligation after intraperitoneal injection of 10% chloral hydrate anesthesia,incision on the left pubis, blunt dissection under the left kidney to the left ureter, 4-0 with silk thread ligation two up and down, on a pole under the left kidney, cut off from among two ureters,step by step a closure in abdominal cavity. Control line two operations during this period, the operation environment and method with the remaining four scores, but only stripping dirty capsule after each open abdominal cavity, abdomen.The first 2 days after the surgery, for all his pups lavage: cordyceps group(1.2 mg/g/d dissolve in the volume of distilled water to fill the stomach). Control(such as volume of distilled water to fill the stomach), model group(such as volume of distilled water to fill the stomach), stilbene Huang Bao renal party group of the stilbene Huang Bao renal party 4 mg/g/d dissolve in volume of distilled water to fill the stomach, the joint group of 4 mg/g/d stilbene Huang Bao renal party + 1.2 mg/g/d cordyceps mycelium powder,soluble in volume distilled water to fill the stomach. Surf and 18:00 each lavage once a day.According to a new calculate weight dose every week. 21 days after surgery in each group to be put to death, only 10 anesthesia with blood collection needles after caesarean section in the abdominal aorta in pursuance of 2-3 ml, measuring the content of serum creatinine and urea nitrogen, quickly remove obstruction side kidney and observe its appearance,Half saline washafter kidney tissue soaked in 10% foer Ma Linzhong curing, light microscope to observe the pathological change, immunohistochemical detection of PAX2 expression.Experimental result:1.Compared with model group, stilbene Huang Bao renal party group, the joint group and Chinese caterpillar fungus were general condition is good, is better than that of model group were signs.2.Compared with model group, stilbene Huang Bao renal party group, the joint group and Chinese caterpillar fungus were serum creatinine and urea nitrogen levels were lower than that of model group. Serum creatinine and urea nitrogen content including united group were superior to other groups.3.To the obstruction of bilateral renal tissue optical microscope observation: visible ball control, renal tubular interstitial hyperplasia of not seen. Model group(figure 2) visible kidney ball bureau du or all of the hardening, heavy ball mesangial cells and matrix hyperplasia,thickening of basement membrane, tube expansion, contraction, interstitial has a large number of inflammatory cells infiltration, with fibrous tissue increased, interstitial fibrosis. Chinese caterpillar fungus(figure 3) and yellow stilbene group(figure 5) minor glomerular sclerosis of focal, part of the small tube slight expansion, mesangial proliferation, mild fibrosis, with inflammatory cells infiltration. Joint group(figure 4) mild hyperplasia of interstitial cells, part of the tubular expansion, thinning of the wall, a small amount of inflammatory cells infiltration.4.Compared with model group, immunohistochemical semi-quantitative analysis showed that stilbene Huang Bao renal particle group and Chinese caterpillar fungus, renal tubular PAX2 expression of the joint group were less than model group.Experimental conclusion:1.Stilbene Huang Bao renal party can improve the living condition of the renal failure were.2.Stilbene Huang Bao renal party can improve renal failure rats kidney function.3.Stilbene Huang Bao renal party can reduce renal failure were renal pathological damage,reduce the damage of the renal tubules, renal interstitial fibrosis of antagonism.4.Stilbene Huang Bao renal party can cut kidney failure were kidney PAX2 expression,reduce extracellular matrix composition and slow the progression of renal interstitial fibrosis,speculated that it may be for the stilbene Huang Bao kidney pellet is one of the mechanism of action of prevention and treatment of renal failure, may provide the basis for clinical treatment of children's kidney failure.And joint group(figure 10) cut PAX2 protein expression of the most obvious.