The Designed Of The PH-sensitive SOD1-DNA-CPP Aggregation

Gene therapy is a broad term that encompasses any strategy to treat a disease by introducing an exogenous gene into cells of an affected person, and providing effectively targeted and safe carriers is a major challenge for gene delivery. The idea of protein-based therapy is to repair the protein mediated diseases through the delivery of normal protein in vitro or in vivo. The pH-Sensitive carriers are potential components of DNA and protein.We try to designe the pH-sensitive SOD1-DNA-CPP aggregation in order to load SOD1 and DNA into cell. DNA acts as a template for accelerating the formation of SOD1 aggregation and is incorporated into SOD1 aggregation under acidic conditions.This process and mechanism is similar to the self-assembly of some virus particles, by which we can designe one kind of the pH-Sensitive virus-like gene- and SOD1-delivering nanoplatforms.On the other hand, CPP?SOD1 and DNA can form another SOD1-DNA-CPP aggregation under neutral conditions.We find that the SOD1-DNA aggregation which is formed under acidic conditions will depolymerize to be the naked DNA and SOD1 under neutral conditions. The SOD1 still has the same activity and the secondary structures. CPPs are directly incorporated without covalent conjugation to SOD1-DNA complexes and may improve its ability to enter the cell. We can get more stable SOD1-DNA-CPP aggregation by extending the reaction time, however it has side-effect on the activity and the secondary structures of SOD1. It may be a potential carrier of DNA and SOD1.We make an attempt at using CPP and SOD1 to compact DNA to get SOD1-DNA-CPP aggregation under neutral conditions. It has little effect on the activity and the secondary structures of SOD1.The SOD1-DNA-CPP aggregation which is formed under neutral conditions is more stable and has less damage on SOD1and pBR322 DNA than the previous. It may be another potential pH-Sensitive gene- and SOD1-delivering nanoplatforms.