The Expression Of Mast Cells Chymotrypsin In Pathological Scar Tissue

Objective:To explore the expressed activity of mast cells chymase exist in human keloid tissue or hypertrophic scars. To further study the foundation mast cell chymase in wound repair.Methods:The number of mast cell and chymase expression in keloid or hypertrophic and normal skin were assessed by immunohistochemical methods. Quantitative real-time PCR and Radio immunity method were conducted to measure the change of mRNA expressed activity of chymase in keloid and normal skin tissue. Results: Compared with normal skin tissue, the number of mast cells and chymase expression were higher in keloid tissues,hypertrophic scar [(10±0.25)/hp, [(12±0.50)/hp vs (4±0.22)/hp, and (6.2±0.23) mm2 vs (2.5±0.12)mm2, the (Ps<0.01)].Strong expression of mast cells stained chymotrypsin. The results of RT-PCR showed mast cell mRNA expression levels and chymase mRNA were also significantly higher in keloid tissues (P<0.05). Conclusion Mast cells and mast cell chymase CD117 immunohistochemical staining was significantly more than the normal skin tissue, strong expression of mast cells stained chymotrypsin. The chymase of mast cells exists in keloids and expressed activity of that were statistically higher than those of normal skin tissues.