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Study Of Cuttage Propagation Technology And Low Dose Stimulation Effect Of Acca Sellowiana Berg

Posted on:2019-02-02Degree:MasterType:Thesis
Country:ChinaCandidate:Y L ZhongFull Text:PDF
GTID:2333330548950408Subject:Biology
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Acca sellowiana Berg is the evergreen woody plant of Magnoliopsida Rosaceae?Myrtaceae?Feijoa Berg or Acca Berg.In order to improve the quality of the seedlings,the studies on the cuttage propagation and seedling test were done separately.In the early stage,Study on physical radiation on stimulation growth of Acca sellowiana Berg seedings:?1?the study of physical radiation(Ar+ion implantation,X ray,60Co-?radiation)to promote the branches survival;?2?the study of physical radiation(X ray,60Co-?radiation)promoted seed germination and seedlings survival.?1?The effect of different variety,plant growth regulator and matrix on the rate of Acca sellowiana Berg cuttings rooting.the sequence of influential factors to the rate of cuttings rooting was variety>plant growth regulator>matrix,the sequence of influential factors to the length of roots was matrix>plant growth regulator>variety,the sequence of influential factors to the number of roots was variety>matrix>plant growth regulator.The semi lignified cuttings of'Gemini'variety that were soaked in 1000mg/L ABT for two minutes,Using humus+vermiculite as the matrix,produced the best results for rooting.The average rooting rate was 70.00%,the length of root was 8.85 cm and the mean number of root was 10.22 on average.?2?The effect of physical radiation on the survival rate of Acca sellowiana Berg branches.The survival rate of the branches was inhibited by Ar+ion implantation.The epicormic branches were suitable for the 10 Gy of X ray radiation treatment,the survival rate was 16.77%,which was 106.77%of the control group;the fully lignification branches were suitable for the 6 Gy of X ray radiation treatment,the survival rate was 56.67%,which was 106.00%of the control group;the semi lignification branches were suitable for the 5 Gy of60Co-?ray radiation treatment,the survival rate was 53.33%,which was106.00%of the control group.?3?The effect of physical radiation on physiological characteristics of Acca sellowiana Berg branches.The epicormic branches was irradiated with 10Gy of X ray radiation,the activity of oxidase?SOD,POD,CAT?and the content of soluble sugar and water increased in leaves.The fully lignification branches was irradiated with 6 Gy of 60Co-?ray radiation,the activity of oxidase?SOD,POD,CAT?and the content of soluble sugar increased in leaves,all achieved peak value,but the content of water was inhibited in leaves.The semi lignification was irradiated with 5 Gy of 60Co-?ray radiation,the activity of oxidase?SOD,POD?and the content of soluble sugar and water increased,but the CAT activity was inhibited in leaves.?4?The effect of physical radiation on seed germination and seedlings survival of Acca sellowiana Berg.The 25 Gy of X ray and 60Co-?ray radiation increased the germination rate of‘Unique'seed,inhibited of the survival rate of seedlings.20 Gy60Co-?ray radiation increased the seed germination rate of‘Unique'?‘Coolidge'?‘Gemini'?‘Opal Star'?‘David',increased the survival rate of'Gemini'seedlings to 97.68%,Inhibited the survival rate of‘Unique'?‘Coolidge'?‘Opal Star'?‘David'seedlings.The sequence of sensitivity of five varieties:‘Gemini'>‘David'>‘Coolidge'>‘Opal Star'>‘Unique'.?5?The effect of physical radiation on the growth of seedlings.The 25 Gy of X ray radiation promoted the stem growth of‘Unique'seedlings,the 25 Gy of60Co-?ray radiation promoted the stem and leaf growth of‘Unique'seedlings,Inhibited the root growth.The 20 Gy of 60Co-?ray radiation promoted the stem and leaf growth of seedlings of‘Unique'?‘Coolidge'?‘Gemini'?‘Opal Star'?‘David',promoted the root growth of seedlings of‘Unique'?‘Gemini'?‘Opal Star',Inhibited the root growth of seedlings of ‘Coolidge' and ‘David'.
Keywords/Search Tags:Acca sellowiana Berg, cuttage, propagation technology, low dose, stimulating effect
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