TCR Signaling Pathway In Orange-spotted Grouper And Expression Patterns Of Its Immune-related Molecules Involved In The C.irritans Infection

T cell activation is a complicated process accompanying with the activation of T cell receptor(TCR)signaling pathway,which is not well described in teleost fish.The initiation of this pathway depends on the interaction of membrane TCR co-receptors(e.g.CD4/8,CD3 and CD45)and a series of cytoplasmic protein tyrosine kinases(e.g.Lck,Fyn and ZAP70).Cyptocaryon irritans is a ciliate pathogen of marine fish white spot disease causing huge economic lost in marine aquaculture.This parasite can infect fish gill and skin and is considered to be a good pathogen model for fish gill and skin mucosal immunity.Our previous studies showed the locally mucosal antibody response was important for fish defense against this parasite.While how TCR signaling pathway involved in T cell activation to help B cell activation in C.irritans infected fish is still not known.In the present study,we cloned a grouper TCR co-receptor gene EcCD3?(537 bp)and its three kinase genes,including EcLck(1512 bp),EcFyn(1605 bp)and EcZAP70(1893 bp).Homology analysis showed that they all shared the highest identity with corresponding genes from Takifugu rubripes(EcCD3? 41%,Ec Lck 88%,Ec Fyn 98% and EcZAP70 93%),and their conserved motifs involved in the signaling transduction were analyzed.The tissue distribution analysis showed these four genes were high expressed in thymus,and it is interesting to find their comparative high expression in skin,gill and midgut mucosal immune tissues.In C.irritans infected grouper,the expression of three TCR co-receptors(EcCD4-1,EcCD3? and EcCD45)and three kinases(EcLck,EcFyn and EcZAP70)was tested in skin,gill,head kidney and spleen at 0,6 h,12 h,24 h,2 d,3 d,5 d and 7 d.All six genes were significantly up-regulated in skin at most tested time points,which indicate the possibility of skin local T cell activation to support the local antibody response.Compared to three TCR co-receptors,significantly up-regulation of three kinases were seen in the spleen,and the spleen fold changes of these three kinases were much higher than head kidney,which indicates spleen maybe the major systematic immune organs for T cell activation in C.irritans infected fish.