Genetic Diversity Analysis And Restorer Gene Mapping Of The New Resources Of Cytoplasm Male Sterile Of Hybrid Rice(CMS-FA)

Fujian wild rice cytoplasm resource of hybrid rice(CMS-FA)that bred by Fujian Agriculture and Forestry University is a new genetic system,referred the new source of hybrid rice,cytoplasm type,maintainer source,relationship between restorer and maintainer are different from three-line hybrid rice WA and HL.Deeply studying male sterility restore genetic features,and systematic analysis of genetic diversity of this new hybrid rice is one of the main contents about utilizing hybrid rice with the new resource of CMS.In this paper,Using both agronomic traits and the SSR molecular markers,the genetic diversity of germplasm resources of CMS-FA is systematically analyzed.Moreover,the sterility restorer gene of CMS-FA was mapped.The major results are as follows:1.By the investigation and analysis on the 8 agronomical traits of 90 germplasm resources of CMS-FA.Using hierarchical cluster analysis,the 90 germplasm resources were divided into three groups when the chi-square distance was 5.87 which Consists of 66,23 and 1 materials respectively.Moreover,when the chi-square distance was 4.20,group I was divided into two subgroup which Consists of 25 and 41 materials respectively.Group Ⅱ was divided into two subgroups when the chi-square distance was 4.20.The first subgroup included 20 resources.The second subgroup only included 3 resources,the 59 main parent resources were divided into two groups when the chi-square distance was 5.10 which Consists of 36 and 23 materials respectively.All sterile lines as well as some of restorer lines of CMS-FA are divided in Group Ⅰ,while group Ⅱ includes most of the restorer lines.Each group has the obvious morphological differences and the subclasss is smaller.2.Using 24 SSR primers recommended by CNRRI,the genetic diversity and genetic distance of germplasm resources of CMS-FA is systematically analyzed.22 primers displayed well in polymorphism,a total of 87 allelic loci were detected.The number of alleles per locus(A)ranged from 3 to 6.Every primer pairs offered 3.95 allelic loci on average.The value of allelic polymorphism inform ation content(PIC)ranged from 0.1865 to 0.6859,on an average of 0.4415.SHAN cluster analysis showed that the 90 resources could be classified into two types when the genetic similarity coefficient was 0.81,the 90 resources could be classified into 2 types which Consists of 89 and 1 materials respectively.Moreover,all resources could be classified into 4 types when the genetic similarity coefficient was 0.75.Type I contained 87 resources,this type contain virtually all of the tested resources,the remaining three resources were classified into three groups.These results have some differences with the morphological classifications.The results can not be completely representative of the genetic diversity of CMS-FA probably due to the numbers of agronomic traits were too small.3.Using the results of SSR,analysis principal component and estimate genetic diversity parameters of 90 germplasm resources.Principal component analysis showed that the first PCA accounted for 95.56%of the total variation observed.Most of the resources distribution is very intensive in groups apart from individual materials(54 and 88).This result is a certain degree of clustering used SSR molecular markers.The main 59 parental resources were divided into three groups by SHAN cluster analysis,analysis values of 4 genetic diversity parameter of number of alleles,effective number of alleles,genetic diversity and diversity index.The performance of the genetic diversity level of the three groups was Ⅰ>Ⅱ>Ⅲ.Parental resources of CMS-FA have high overall genetic diversity index,different groups have relatively low genetic diversity index and high level of genetic differentiation.4.genetic analysis according to the F2 population of R402-6/Acc8558 indicated that male sterility restorer gene of CMS-FA was controlled by a single dominant gene,and named the gene Rf-FA temporarily.Using SSR molecular markers(BSA)method,The restorer gene was mapped on chromosome 10,Rf-FA is located on the same side of the two SSR markers RM467 and RM596,and the genetic distance between the two markers were 13.5cM and 5.7cM respectively.