Transcriptomic And Proteomic Analysis Of Banana Response To Salt Stress

Banana is one of the most competitive agricultural products in China, but it belongs to salt-sensitive plants. In production, the most suitable area of banana cultivation in Hainan,Guangdong Zhanjiang and other regions. With the increasing salinization of land, which hinder the sustainable development of banana industry. While the transcriptome and proteome studies of banana responses to salt stress have not been reported. In this study,transcription and proteome analysis of China’s main varieties Brazilian banana (Musa paradisiaca) roots and leaves at 60 mmol / L NaCl artificial simulated salt stress for 0, 12,24 h by RNA-Seq and iTRAQ technique, which was expected to reveal the molecular mechanism of banana response to salt stress and to lay the foundation for banana molecular breeding.The results are as follows:1、The different tissues of Brazilian banana have different ways to response to salt stress. Salt treatment for 12 h, there were 542, 924 up-and down-regulated genes of roots;2938, 2727 up-and down-regulated genes of leaves. In 24 h, there were 205, 334 up-and down-regulated genes of roots, respectively; 141 and 234 up-and down-regulated genes of leaves, respectively. In 12 h/24 h, the roots had 242 up-regulated genes, 1116 down-regulated genes, while leaves had 2014 up-regulated genes and 2753 down-regulated genes. From the overall analysis, the leaves differentially expressed genes were more than the roots and most of them were up-regulated, but the roots were just the opposite. Leaves under salt stress for 12 h the differentially expressed genes were more than 24 h. While the roots under salt treatment for 24 h differentially expressed genes more than 12 h, most of them were down-regulated.2、Combined with GO and KEGG differentially expressed genes enrichment analysis,the function of differential expressed genes were mainly involved in signal transduction,hormone signal transduction, reactive oxygen scavenging, secondary metabolites and energy metabolism and so on. In addition, the differential expressed genes of leaves were mainly involved in signal transduction and hormone signal transduction, while roots were sucrose and starch metabolic pathways.3、Transcriptional analysis found that MYB, GPCR, HAK, NAC, NHX1, bZIP,WRKY and other salt stress transcription factors.4、Ten differentially expressed genes of roots and leaves were selected for qRT-PCR verification.The results of the verification were consistent with those of RNA-Sep sequencing, which proved that the sequencing results of the transcriptome were reliable.5、Under salt treatment for 12 h there were 162, 282 up- and down-regulated proteins of root. 184, 212 up- and down-regulated proteins of leaves. In 24 h, the roots had 205 up-regulated proteins, 334 down-regulated proteins. While leaves had 141 up-regulated proteins, 170 down-regulated proteins, respectively. In 12 h/24 h, the roots had 154 up-regulated proteins, 139down-regulated proteins, with the leaves had 213 up-regulated proteins, 234 down-regulated proteins. From the overall analysis, the roots and leaves in the process of response to salt stress down-regulated proteins than the up-regulated proteins.6、GO and KEGG analysis of differentially expressed proteins function was show that these proteins were mainly involved in processes such as antioxidant-related proteins,signal transduction, protein translation processing and degradation, energy and transport,cell division, structure and cytoskeleton, defense, protein synthesis and processing and degradation.Among them, the differentially expressed proteins of leaves were mainly involved in the process of photosynthesis, metabolism and antioxidant processes. Most of the differential expressed genes of roots were involved in the process of protein synthesis and cell division.7、Proteome and transcriptase association analysis showed that coverage were low between protein and gene . Correlating to differentially expressed proteins at the same trend with differentially expressed genes of banana were β-galactosidase,oxygen-enhanced protein, heat shock protein, tubulin, chitinase, ABC transporter, LRR receptor kinase etc. Correlating to differentially expressed proteinsat the opposite trend with differentially expressed genes of banana such as chlorophyll a / b binding protein,histone, malate synthase and so on.In summary, transcriptome and proteome analysis the molecular regulation mechanism of Brazilian banana response to salt stress. And screened with salt-related functional genes and proteins, indicating that the Brazilian banana has salt tolerance.Which provided some theoretical basis for cultivating new varieties of salt-tolerant banana and study on salt tolerance mechanism of Banana.