Role Of WAX INDUCER1/SHINE1 In Osmotic Stress Response In Brassica Napus And CLCc3 Cloning In Rice

Waxes consist of very long chain fatty acids(VLCFAs)and their derivatives.Waxes are deposited in the aerial surfaces of different plants and are important components to decrease water loss from non-stomatal transpiration.Waxes also function as a barrier against pathogen and insect attack,and are essential for plants to adapt adverse environment.Brassica napus(B.napus)plants are frequently suffered from different stresses including drought,salinity,waterlog,and pathogen attack.It is great beneficial to develop high resistant crop plants with enhanced wax accumulation due to it multiple effects.However,it is relative limited about the regulation of wax biosynthesis in crop plants.In this study,expression profiling revealed that WAX INDUCER1/SHINE1(BnWIN1)is expressed in various tissues with higher level in flowers,flower buds,siliques,leaves,but it is lower in roots.To explore the role of BnWINl in stress response,BnWINl was cloned and overexpressed in B.napus.BnWIN1-overexpression(OE)plants accumulated more waxes as observed under SEM and exhibited more tolerance to salt stress.OE plant contained more chlorophyll under salt stress and grew better than WT plants.In addition,the seeds of OE plants also accumulated more oil than WT.And seed oil content in OE was not significantly different from WT under salt stress.The results suggest that BnWIN1 enhanced overall lipid anabolic process without at the expense of oil accumulation even enhanced wax synthesis under salt stress.BnWIN1 is localized to nucleus as visualized under laser confocal microscope.The results provide a potential application of BnWIN1 in crop plant breeding.In addition,the gene encoding Chloride Channel c3(CLCc3)was cloned from rice and was constructed for the overexpression,GFP fused protein,GUS reporter.Moreover,the transgenic plants for these constructs were generated.The clcc3 mutant was isolated and its genetic OE was also generated for functional characterization.