Key Techniques For Industrial Propagation In Spathiphyllum 'Mojo'

Key techniques including section and sterilization of explants,selection of basic media,selection of combination and ratio of plant regulators in order to promote induction of calli,differentiation of adventitious buds and rooting of sterile seedings in the process of industrial propagation of Spathiphyllum were studied systematically by cultuer with leaves and petiole and stems as explants in in cultivars of Mojo,effects of light conditions on induction of calli was studied.The technical standards and systems was developed and perfected to overcome and solved problems in industrial propagation,which will provide technical suppors in industrial propagation in Spathiphyllum.The main results were as follows:1.The suitable method for sterilization of explants was that the explants were firstly washed by flowing water for about 0.5 hour,then soaked with 75%alcohol for 30 seconds,and transferred to containing 0.1%mercury chloride for sterilization(8minutes for leaves,and 9minutes for petiole),after removal of 0.1 mercury choride,washed with sterile water for 5 times.2.MS+6-BA 1.0mg/L+2,4-D 0.8mg/L+agar 5.5g/L+cane sugar 30g/L was the best culture medium in all the medium treatments for callus induction of Sathiphyllum.3.Culture under scattering light 10001x for 13 hours,then culture under dark for 11 hours was beneficial to induction of calli;culture under dark only was not beneficial to induction of calli and adventitious buds.4.MS+6-BA 1.0mg/L+IBA 0.4mg/L was the best culture medium for adventitious buds induction of Spathiphyllum.5.MS+6-BA 1.0mg/L+NAA 0.8mg/L was the best culture medium for adventitious buds multiply of Spathiphyllum.6.The tissue culture plant of Spathiphyllum was easy for rooting.The suitable auxins was IB A 0.4mg/L.In this concentration the quality and number of the root was best and the rooting rate was 100%.The results inducted that added the active carbon nearly have positive affects for rooting.7.When transplanting,the culturing room were cultured for 7 days and 4 days in the greenhouses,respectively,then seedlings were hardened for days by opening caps of bottles and adding little water into the bottles cultured for 3 days,which would save costs and increase survival rate to 93%.The optimal transplanting medium to Transplant Spathiphyllum was composed of sand plus aquatic weeds and peat soil(1:1:2).8.0.1%urea is the best fertilizers in the transplanted tissue culture seedling.250mg/L of GA,for Spathiphyllum optimum concentration of flowers.9.The main industrial-scale micropropagation technical chain of Spathiphyllum 'Mojo' was:the establishment of sterile-plantlets system from the explants?the bud or rhizome proliferation culture?plantlet rooting and growth acclimatization?transplanting onto the aperture disks?spotting?sales.The main technical parameters were:the sterile-plantlets system from the explants was under the conditions of 0.1%HgCl2sterization for 9min;the proliferation coefficient was 5.47 on the MS+1.0 mg/L6-BA+0.4mg/LIBA);and the rooting medium was MS+0.4mg/LIAA +0.1%urea and the rooting rate was 100%;and the survival rate of transplanting was more than 97%(plus aquatic weeds and peat soil 1:1:2).According to the technical parameters through experiments,taking the annual output all the costs,including the cultural medium preparation costs,the total electricity costs,the total water charges costs,the total consumable costs,the total depreciation costs and the transplanting costs,were 443333.2Yuan,and according to the market price of 0.62 Yuan,the total profit was 195439.13 Yuan.It was found that the charge for electricity was the highest with the cost of 187395 Yuan,which accounted for 42.27%of the total costs.The labor costs were the second with the cost of 158805 Yuan,accounting for 35.82%of the total costs.