Study On Regulation Of PPAR Gene Transcription By FATP1 Promoter In Cattle

Fatty acid transporter 1(FATP1)promotes the long chain fatty acids into cells and coordinates the intake of free fatty acids,thereby affecting lipid distribution and deposition.Studies have shown that FATP1 can promote the deposition of adipose in the relevant tissues.It can be involved in lipid metabolism and in energy metabolism.Peroxisome proliferator-activated receptor gamma(PPAR?)as a protein gene can be mainly found in adipose tissue cells.It is involved in adipose deposition regulation,lipid metabolism and adipocyte differentiation.In this study,the expression of PPAR? gene was detected by qRT-PCR in the tissues of heart,liver,spleen,lung,kidney,dorsal longissimus dorsi and adipose in Guanling cattle,Sinan cattle,Liping cattle and Weining cattle.Three fragments with high activity of FATP1 gene promoter and the CDS region of PPAR? gene were cloned in Guanling cattle.pEGFP-N3-FATP1-Pn-PPAR?eukaryotic expression vector was constructed and then transfected into mouse 3T3-L1 adipocytes and bovine primary adipocytes.By observing the strength of green fluorescence,it was confirmed that the promoter of FATP1 gene could drive the PPAR? gene to express in the cells.The expression of PPAR? gene in mouse 3T3-L1 and bovine adipocytes were detected by q RT-PCR.The effect of FATP1 gene promoter on transcriptional regulation of PPAR? gene was further verified.The main results are as follows:(1)PPAR? gene was expressed in four different tissues of local cattle in Guizhou.The expression in the tissue is different and has adipose tissue specificity.The expression was also different in part tissues of different cattle resources.(2)FATP1-P8,FATP1-P7 and FATP1-P4 promoter fragment had positive effects on PPAR?gene expression in mouse 3T3-L1 cells and bovine primary adipocytes.And the effect of FATP1-P4 promoter fragment was most significant.