| Rapeseed(Brassica napus L.),is one of the world’s major oil crops,the main source of protein feed,edible oil and industrial raw materials.The main producing areas of the Yangtze River rape are the largest rapeseed production belt in the world.The problem of pest damage is generally serious,which restricts the growth and production of rape.Therefore,the application of herbicide is necessary.It Is the development of modern agriculture,efficient and efficient measures to control grass.glufosinate as one of the most widely application scope of herbicides in the world,which has advantages of its broad spectrum,high efficiency,low toxicity and other characteristics wide,has a broad application value and market competitiveness.Although the impact of herbicides on non-target organisms has been studied by some scholars,the effect of residual glufosinate on the transplanted rapeseed seedlings has not been reported.Therefore,in this study,two rape lines of Zhongshuang 11 and D148 were used to study the glufosinate stress test.In September 2015 were artificial weeding,glufosinate application and the rape was transplanted after 4 days.Among them,glufosinate was set at 3 concentration levels: 0g·hm-2,450 g·hm-2 and 90 g·hm-2,a total of six treatment levels.Using physiological and biochemical analysis method,studied the effects of glufosinate administration and non-application,recommended dosage and overdose on the assimilation of nitrogen assimilation and chlorophyll biosynthesis were studied by physiological and biochemical methods.At the same time,The effects of two rape lines on the membrane lipid oxidation system was studied.The main results as follows:1.The recommended dose and excess of glufosinate in soil were inhibited by root length,plant height,fresh weight and dry weight of rapeseed seedlings before transplanting.The recommended dose of glufosinate resulted in a decrease in plant height and a decrease of 3.00%.Excessive application of glufosinate inhibited the plant height of D148 and Zhongshuang 11 rape seedlings,with the decrease of 6.62% and 18.98%,respectively.The recommended dose of glufosinate had no significant effect on the root length of D148 and Zhongshuang 11 rape seedlings.In the case of overdose,the root length of Zhongshuang 11 seedlings was inhibited by 13.9%.The recommended dose or excess application of glufosinate herbicide inhibited the fresh weight and dry weight ofD148 and Zhongshuang 11 rapeseed seedlings,and the effect of the recommended dose of glufosinate on the fresh weight and dry weight of rapeseed seedlings was less than that of overdose.2.The activity of SOD and CAT in rapeseed seedlings was significantly higher than that in the control group(P <0.05).The recommended dose of glufosinate enhances the activities of SOD and CAT in leaves and roots of two rape seedlings.The activity of SOD and CAT in the leaves and roots of D148 seedlings was increased by over-spraying,while the activity of SOD and CAT in the leaves and roots of the leaves were enhanced by the enhancement-inhibition-enhancement.The POD activity of leaves and roots of two rape cultivars decreased significantly when spraying the recommended dose of glufosinate and overdose,The effect of overdose on the POD activity of leaf and root of two rape varieties was higher than that of the two groups,and the effect was over as follows.The content of malondialdehyde(MDA)in leaves and roots of transplanted rape seedlings was significantly increased by different concentrations of glyphosate,and the effect of spraying the recommended dose of glufosinate on the content of malondialdehyde in rape seedling was less than that of overdose.The content of proline in the leaves of two rape seedlings was higher than that of the roots under the same dose,and the content of free proline in the leaves and roots of the rape seedlings increased with the increase of the concentration of glufosinate.3.Compared with the control,the GS and GOGAT activities of the leaves and roots of the two rapeseed lines decreased both the recommended dose and the overdose,and the GS and GOGAT activities of the leaf were slightly stronger than those of the root organ.The activities of GDH in the leaves and roots of both rape seedlings showed an increasing trend,and the effect of the recommended dose of glufosinate on the three key enzymes of nitrogen metabolism was less than that of overdose.The content of NH4+ and the total amount of free amino acids in D148 and Zhongshuang 11 were significantly increased after treatment with glufosinate,and the effect of the recommended dose of glufosinate on the leaf and root of rapeseed seedlings was less than that of overdrafts After 70 days,the effect of residual glufosinate on the content of NH4+ and the total amount of free amino acids in the leaves and roots of Brassica napus were not significant.In contrast to the results of total NH4+ and free amino acids,the soluble protein content and total nitrogen content of the leaves and roots of the two rape treatments were significantly lower than those of the control,and the effect of the recommended dose of glufosinate on the soluble protein and total nitrogen content of rapeseed seedlings was less than that of overdose. |
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