Optimal Supplemented Amount And Ratio Of RPMet、RPLys In The Ration Of Growing Sika Deer

The objective of this study was to determine the proper supplementation levels and ratio of rumen protected methionine(RPMet)and rumen protected lysine(RPLys)in growing Sika deer fed low protein diet,high protein diet,low protein diet supplemented with RPMet and RPLys and high-protein diets supplemented with RPMet and RPLys separately,thus reducing the amount of protein supplement comsumption and nitrogen emissions in deer farming practice.Experiment 1: The stability of commercail rumen protected amino acid(RPAA)was tested.The results confirmed that the remaining amount of RPMet and RPLys in artificial saliva for 24 h was 87.1 and 85.83% respectively;while in the buffer of PH6.6 was 82.63 and 82.41% respectively;in the buffer of PH5.4 was 79.25 and 79.04% respectively;in the buffer of PH2.4 was 1.13 and 2.61% respectively.Overall,the product performance in rumen escape seems very well,and easy to release at p H2.4.Experiment 2: Twenty-five healthy sika deer with similar live weight,including 15 stags and 10 does,were randomly divided into five groups,namely groups Ⅰ,Ⅰ,Ⅰ,andⅠ Ⅰ.Animals in groupⅠwere fed a concentrate supplement contained 21.02% of crude protein,in group Ⅰ,28.21%,animals in groups Ⅰ,IV and V were fed a concentrate supplement the same to group I plus 0.08% RPMet and 0.16% RPLys,0.125% RPMet and 0.28 % RPLy;0.2% RPMet and 0.5% RPLys,respectivelyThe results showed that the daily weight gain of animals in group was significantly Ⅰhigher than that of other four groups(P <0.05);The daily weight gain of animals in group Ⅰwas significantly higher than that of groups I,Ⅱ,Ⅲ(P <0.05);The daily weight gain among groupsⅠ,Ⅰand was not significⅠ ant(P>0.05).There were no significant differences in the digestibilities of crude fat,crude protein and organic matter among groups(P>0.05).Concerning the blood biochemical indexes,blood glucose level of group was significantly Ⅰhigher than that of groupsⅠand Ⅰ(P < 0.05),thoseamong other groups was not significant(P > 0.05).For blood physiological indexes,white blood cell levels of group was Ⅰsignificantly higher than that of groupsⅠ,Ⅰ,(Ⅰ P < 0.05).hemoglobin level of animals in group was siⅠ gnificantly higher than that in groups Ⅰ,Ⅰ,(Ⅰ P < 0.05),in groupⅠwas significantly higher than that in group Ⅰ,(Ⅰ P < 0.05);w-scc of animals in group was Ⅰsignificantly lower than that in groups and(Ⅰ Ⅰ P < 0.05);red cell distribution width(CV)for group was significantly higher than that for group(Ⅰ Ⅰ P < 0.05).The free amino acid content in plasma varied among groups.Methionine level for group was significantly higher than that Ⅰfor groupsⅠ,Ⅰ,(Ⅰ P < 0.05);The content of cystine for groupsⅠand were significantly Ⅰhigher than that for groups and(Ⅰ Ⅰ P < 0.05),that for group was significantly higher than Ⅰthat for group Ⅰ(P < 0.05).The costs per kilogram of concentrate supplement for groups Ⅰ,Ⅰ,Ⅰ and Ⅰwere 0.30,0.28,0.48 and 0.83 yuan higher than that for group Ⅰ.Experiment 3: Twenty-three healthy sika deer with similar live weight were divided into three groups,namely grous Ⅰ,and with 7,8,8 animals separately.Each group contained Ⅰ Ⅰthree does,the others were stags.Animals in each group were fed a basal concentrate supplement containing 28.21% of crude protein adding 0.08% RPMet and 0.16% RPLys,0.125% RPMet and 0.28 %RPLys,and 0.2%RPMet and 0.5%RPLys for groups I,II and III,respectively.The results showed that There were no significant differences in the daily weight gain,digestibility of nutrients and blood biochemical indexes among treatments(P>0.05).In the blood physiological indexes,P-LCR for group was significantly higher than that for ⅠgroupⅠ(P<0.05).The contents of aspartic acid and glycine in plasma for group I were significantly higher than those for groups and Ⅰ Ⅰ(P<0.05).Experiment 4: The animals used in Experiment 3 were used in this experiment.Grouping was the same as experiment 3.Animals in each group were fed a basal concentrate supplement containing 21.02% of crude protein adding 0.125% RPMet and 0.28% RPLys,0.125% RPMet and 0.21 %RPLys,and 0.09375% RPMet and 0.28% RPLys for groups I,II and III.The results showed that subtracting part of RPMet and RPLys reduced the daily weight gain of sika deer but did not differ significantly(P>0.05).Subtracting part of RPMet and RPLys reduced the digestibility of crude protein in growing sika deer,but did not reach a statistically significant level(P>0.05).The levels of total protein in the blood biochemical indexes for group was significantly higher than that forgroup Ⅰ Ⅰ(P<0.05).In the blood physiological indexes,the width of red blood cell for Group was Ⅰsignificantly lower than that for group Ⅰ(P<0.05);the platelet distribution widthfor group Ⅰwas significantly lower than that fro group Ⅰand group Ⅰ(P<0.05).Subtracting part of RPMet and RPLys had no significant effect on plasma free amino acid concentration(P>0.05).