Effect Of Wax On Drought Resistance Of Mutant MB-1-5 And Primary Of Waxy Gene In Barley

Drought is one of the main factors for crop production,the mechanism of crop drought is very complex,which is also controlled by multiple genes.The wax of cuticleplays an important role in crop drought resistance,but a lot of genes and functions that relate to waxy synthesis and transportare still unknown.In this study,wax mutant MB-1-5 was obtained by EMS,determinate indicators of related to investigate the effect of wax on plant drought resistance by Ganpi 6.At the same time,F2 separation groups was constructed according to parental materials,wax genes were located by SSR markers,find the markers related to goal gene.The main results are as follows:1.There were obvious differences on the leaf sheath and lemma betweeen black barley and wax mutant MB-1-5.Compared with Ganpi 6,the latter has no crystal layer on the leaf sheath and lemma,but there were not obvious difference on the leaf surface of them.2.Chlorophyll content significantly decreased in Ganpi 6 and MB-1-5 with the delay of the drought stress time,the contents of proline,soluble sugar and MDA significantly increased.The contents of proline and soluble sugar in Ganpi 6 were higher than that of mutant,but MDA was lower than wax mutant.3.The combination was constructed by Ganpi6 and wax mutant,F2 isolates regards as the research object.Construction of genetic map using QTL Ici Mapping and finding markers linked to waxy genes by SSR.(1)A total of 53 pairs of polymorphic primers were screened by using Ganpi6 and mutant to draw the genetic linkage map,the map covered 1721.65 c M with an average interval of 32.48 c M.Number of markers mapped onto linkage group varied from 3 to 13.(2)There were 8 wax gene-related sites in 1-7H of barley,which located in 2,4,5,6 and 7H.The site of chromosome 5 was located between EBmac0096 and Bmag0387,It is 14.10 c M distance from the mark EBmac0096.