Mmu-143 Regulate The Expression Of Tyrp1a Gene And Establishment Of Japanese Flounder Albinism Skin Cell Lines

Japanese flounder(Paralichthys olivaceus)is an ecomomically important marine fish,and has been widely cultured in the north of China.The albinism in Japanese flounder aquaculture has become a common phenomenon and influenced its large-scale farming and market value.So,in order to investigate the mechanism of albinism in Japanese flounder from molecular genetics,the albinism and normal Japanese flounder were firstly used for the transcriptome and mi RNA sequencing.In our study,the expression patterns of albinism-related miRNA-mmu-143 and its target gene-Tyrp1 a were studied.We also treated larvae of Japanese flounder with thyroid hormone T3,as a result,the albino ratio increased,and the result of quantitative PCR of the up-regulated expression gene Complement component C8 beta chain(c8b)?Complement component C9(c9)? Myoblast determination protein 1 homolog(myod)and down-regulated expression gene ATP-dependent 6-phosphofructokinase(pfkm)? Melanotransferrin(mfi2)?Somatotropin(gh)are consistent with high-throughput sequencing.In present study,two skin cell lines were sucessfully established from wild type and albino Japanese flounder,JFSK_wt and JFSK_alb.Furthermore,their growth conditions,chromosome karyotype,virus susceptibility,foreign gene transfection and albinism related gene expression were analyzed and compared,which offers a useful platform for fish pathology and skin pigmentation mechanism.The results of this study will provide important basic information to further reveal the molecular mechanism of Japanese flounder albinism.