Comparative Proteomic Analysis Of Potato Tuber Development In Vitro Regulated By Jasmonate Acid

Potato tuber is a nutritional storage organ.The process of potato tuber development is quite complex,which is regulated by many environmental factors and plant hormones.In this study,the potato stolon in vitro were used as experimental material,and the effect of exogenous jasmonate acid(JA)and salicylhydroxamic acid(SAHM)on morphology,physiology and the proteome levels during potato tuberization were investigated,which provides the theoretical foundation for elucidating the regulatory mechanism of JA during potato tuberization process.The results as following1.In this study,exogenous JA influenced the tuber morphology and physiology.The fresh,dry weight,diameter and tuberation rate significantly were increased,whereas the activity of lipoxygenase(LOX)markly was decreased under different JA concentration treatments.These results indicated that JA could help the storage substances accumulate and promote the tuber formation in a certain concentration range.JA also could promote the accumulation of starch.Under 0,0.5 and 5 ?? JA treatments,the starch contents were increased and then decreased.Under 0.5 ?M JA treatment,the starch content was reached the highest value(15.5 mg/g).The content of reducing sugar and soluble sugars were decreased and then increased under JA treatment and reached the lowest value(0.5 mg/g and 2.5 mg/g)under 0.5 ?M JA treatments.Under 0.5 ?? JA and SHAM treatments,the contents of starch were increased and then decreased.Under 0.5 ?? JA and 300 ?M SHAM treatments,the starch content was reached the highest value(22.8 mg/g).And the content of starch was reached the lowest value(5.5 mg/g)under 0.5 ?? JA and 700 ?M SHAM treatments.Under 0.5 ?? JA and SHAM treatments,the contents of reducing sugar were increased siginificantly.Under 5 ?? JA and SHAM treatments,the contents of starch were increased and then decreased.Under 5 ?? JA and 1 ?M SHAM treatment,the starch content was reached the highest value(25.5 mg/g).The contents of reducing sugar were decreased and then increased with 5 ?? JA and SHAM treatments.And the contents of reducing sugar were reached the lowest value(0.4mg/g)and highest value(5.9 mg/g)under 5 ?? JA and 1 ?M SHAM treatment as well as 5 ?? JA and 700 ?M SHAM treatment,respectively.The content of soluble sugars sharply was increased under 5 ?? JA and SHAM treatments.2.Differential expressed proteomics analysis of potato tuber showed that there were 44 differentially expressed proteins were identified under different JA concentration treatment in vitro.These identified proteins were divided into 6 categories,including energy and metabolism,storage and defense,redox,translation,material transport and cellular structure.There were 37 differentially expressed proteins were identified under 0.5 ?M JA and SHAM treatments in vitro.These identified proteins were divided into 5 categories,including energy and metabolism,storage and defense,redox,translation and transportion.There were 31 differentially expressed proteins were identified with 5 ?M JA and SHAM treatments in vitro.These identified proteins were divided into 5 categories,including energy and metabolism,storage and defense,redox,translation and transportion.3.This study found that ADP-glucose pyrophosphate was up-regulated under JA treatment,which could promote the starch accumulation of potato tuber.During potato tuberization,JA regulated the glycolysis pathway,Calvin cycle and the pentose phosphate pathway to promote the conversion of sugars into starch and provide energy,whereas the energy consumptions were reduced by JA in these development processes.JA could enhance the endurance of tuber by removing the reactive oxygen species and toxic C1 compound,such as methanol,formaldehyde and formic acid during tuberizaton.