Effects Of Wetting And Drying Conditions On The Abundance Of Anaerobic Ammonium Oxidation Bacteria And Ammonia Oxidizing Archaea In Soil

The excessive input of artificial synthesis of nitrogen to natural ecosystem had resulted in a number problems of nitrogen imbalance.Study on the control factors of nitrogen cycling functional microorganisms is the hotspot of nitrogen cycle research.The discovery of these two novel nitrogen cycle functional microbes: anaerobic ammonia oxidation bacteria(Anammox)and ammoniaoxidizing archaea(AOA)is a historic breakthrough in the field of nitrogen research in recent years.The alternation of wetting and dryinig condition in soil has an important effect on nitrogen cycle process,in which the response of microorganisms are also different.This paper focuses on the distribution of Anammox and AOA in different environments.A series of culture experiments were conducted to investigate the effects of wetting and drying conditions on the abundance.In addition,the effects on the microbial community characteristics were studied by high throughput sequencing.Fluorescence quantitative PCR was used to determine abundance,high throughput sequencing was performed on Illumina platform.The results show differernt distributions of Anammox and AOA in different environments and diverse responses of Anammox and AOA abundance to alternation of wetting and drying conditions.The compositions of microbial community in paddy soil and sediment were different.The alternation of wetting and drying conditions promoted microbial community succession.The results deepened the understanding of functional microorganisms in nitrogen cycle in natural ecosystems and are of great significance to improve the design and operation management of ecological restoration project such as artificial wetland.