| Wheat gluten protein mainly include glutenin and gliadin.and glutenin determined gluten strength.Glutenin is composed of high molecular weight glutenin subunits(HMW-GS)and low molecular weight glutenin subunits(LMW-GS).HMW-GS accounts for 10%percent of the total wheat gluten protein.but the quality of wheat or gluten quality has a decisive role.Found.create.This study choose from different studies on wheat chromosomes in to the outer material.and carries on the HMW-GS identification.and the analysis of the quality of wheat.finally forecast the Alien chromosome import for the influence of HMW-GS processing quality of wheat.and from the level of molecular genetic encoding amino acid,preliminary explore the relationship between wheat quality and HMW-GS gene.provides useful resources for wheat gluten quality improvement.The main research contents and research results are as follows.(1)Agropyron elongatum disomic addition line and disomic substitution line with common wheat grain protein content.wet gluten content and LASRC.GMP content.and the rheological properties of dough have significant differences.DS1E/1A the bread with a score of 81.5±0.71.significantly greater than the 52±2.83 in CS.Except DS1E/1Dother Agropyron elongatum disomic addition line and disomic substitution line.This experiment also made a correlation analysis of the quality.the study found that bread volume and GMP content.LASRC.mid line peak value.mid line peak width.mid line timex width exists significant correlation.and GMP highest correlation coefficient of 0.938.The study also found that the SCSRC and mid line peak time.mid line peak value.mid line peak width.mid line timex widthxists significant negative correlation.(2)Cloning the gene sequence Agropyron elongatum 1E subunit,the gene of CDS sequence 1512 base pairs,the full-length deduced amino acid sequence of 502 amino acid residues.Gene structure consistent with the structure published HMW-GS subunit gene does not contain introns in the signal peptide,N-terminal conserved region,the central repeat region,C-terminal composition.And cloned HMW-GS gene sequence alignment,phylogenetic tree analysis of the Agropyron elongatum y-type gene and the other logged and the intermediate Thinopyrum gene together with common wheat HMW-GS subunits or x y gene similarity lower test material 1E gene y type HMW protein subunits.(3)Tauschii from wild wheat line 50 hexaploid synthetic grass-D group chromosomes screened for quality,a wide range of genetic variation found among quality traits.Determination by HPLC trace gluten large-mer and glutenin and gliadin ratio index,taking the highest and lowest parts of the respective lines 3-4 further quality analysis showed SE43,SE63 and SE76 and other three lines in the wet gluten aspects of content,grain hardness,lactic acid SRC and knead mix parameters and SE32,SE66,SE75 and SE77 and other strains there are significant differences.(4)Cloning on the synthetic wheat Glu-D1 locus HMW-GS type of x and y-type subunit genes,analysis of the amino acid sequence of HMW-GS gene sequence and the deduced results showed SE43,SE63 and SE76 and other three lines Dy consistent subunit gene sequence,there is a big difference between gene sequences Dy subunit SE32,SE66,SE75 and SE77 and other strains,phylogenetic tree analysis,SE63 subunit of Dy and Quasi Spelt goat grass(A.speltoides)Dy-subunit gene cloned recently.Wheat processing quality of differences and the molecular structure of the HMW-GS gene.we speculate that it is caused by the number of the β-turn sequences and Glutamine.Dy subunit contains four repetitive sequence is less.Compared the two subunits contains the number of glutamine(Q)and its percentage.according to the results of Dx subunit contains the number of glutamine(Q)and molar percentage is higher.It is indicating that repeat sequence in central area of Dx subunit have a better ability to combined with hydrogen bond between each other to form long chains.Dy subunit contains the number of tyrosine(Y)is less.but its percentage is higher.at 6.42%.Experiments found that good quality traits SE63 materials in the central of Dx repeat sequence in β-turn sequences mumber is higher than wheat materials with poor quality.the same conclusion also appeared on the percentage of glutamine(Q)and tyrosine(Y)percentage.the filtered SE63 material of glutamine(Q)and tyrosine(Y)percentage is as high as 39.91%and 39.91%respectively. |
