Characterization Of Inosital Polyphosphate Kinase MoAsp1 In Magnaporthe Oryzae

Rice is a historical native food crops in China,it’s one of the world’s leading food crops,too.Nearly third of the world’s population serve rice as their staple food.It can be seen the importance of rice for people’s livelihood and food issues in the world.Rice blast.one of the world’s three major rice diseases cause by M.Oryzae,is attracking researchers’attention in the world momently by It’s large-scale spread and serious harmfulness.The world mainstream method to prevent the disease is improving rice varieties and chemical control.But because of the widespread dissemination of rice blast fungus,it’s easy to cause type of pathogenic mutations and rapid expansion of resistance groups which makes progress in disease prevention and control strategy of struggling.Therefore,researchers are actively looking for the method for radical cure of rice blast from the pathogenic mechanism,and the detail studies of the pathogenesis of M.oiyzae provide a new strategy for disease resistance breeding and a candidate target for the development of novel fungicides for blast control.In this paper,we focused on functional analysis of inosital polyphosphate kinase associate gene MoASP1 during its development and pathogenicity in M.oryzae,and the main results are showed as follows:Study on yeast discovered that yeast Vipl is a kinase which can phosphorylate inosital polyphosphate(IP6)into an inosital pyrophosphate(IP7),which is intracellular signaling molecules for variety of cell processes.We identified a yeast VIP1 homologous gene MoASP1 with predicted dual domain structure consisting of a conversed N-terminal"rimK"ATP-grasp domain and a conversed C-terminal phosphatase-like domain.Through to the gene knock out mutant characterization analysis,we found that the growth colony of MoASP1 null mutant is reduced compared with wide type Guy11 and complemented strain.Moreover,the asexual development namely conidium produce ability in mutant is affected,and then showed a declining pathogenicity on rice and barley.In addition,MoAsp1 contribute to the resistance to the cell wall and oxidative stress inducer.That means MoAsp1 is important for growth,asexual development and pathogenicity in M.oryzae.It’s reported that yeast Vip1 and some homologous proteins were contributed to fungal growth mainly by regulating MT(microtubule)cytoskeleton which is a conversed feature.Meanwhile,Inositol pyrophosphates generated by Asp1 modulate MT dynamic parameters independent of the central +TIP EB1 and in a dose-dependent and cellular-context-dependent manner.EB1 family is important for plus-end association of numerous +TIPs.In our text,MoASP1 also act as a regulator for β-tubulin under analysis of null mutant,and hence affected polarity growth,then induced to decline of pathogenicity.