| Mitchella undulata which is an ornamental creeping herb belonging to Mitchella,Rubiaceae.It was necessary to protect the species because of its rarity in Zhejiang Province.Domestic research reports on Mitchella undulata were very rare,only occasionary in some floristic surverys,but it was not an independent sdudy of Mitchella undulata.The aim of this paper was to provide references for the presercation of germplasm resources and the later research by studying the tissue culture and rapid propagation technology.1.Different kinds of explants and hormones,as well as culture environment have an important impact on the callus induction rate.Explants played the most significant role in the whole factors,and the band-leaf type was the best.Light culture conditions were more sui Table for the callus induction than the dark conditions,but the light conditions would increase the callus browing rate.Therefor,the dark environment was very necessary at first,then transferred to light conditions to culture continuely when the callus came to morphogenesis stage.The best hormone combinations selected from 6-BA?TDZ?2,4-D?NAA these four plant growth regulators were 0.50 mg·L-1 NAA+2.003.00 mg·L-1 6-BA,which the induction ration even reached 99.56%.2.The result showed that the effect of different explant types and hormones on the induction of adventitious buds was very significant.The best induction medium was MS+0.02 mg·L-1 TDZ+0.10 mg·L-1 2,4-D+0.50 mg·L-1 6-BA.And the best explant was band-leaf type.3.Different hormones showed different results in the proliferation of cluster of buds.6-BA was better than TDZ and KT,while IBA was better than NAA.Thus,the optimum medium was MS+1.0 mg·L-1 6-BA+0.10 mg·L-1 IBA.4.The salt concentration had a great effect on rooting,which showed that low concentration promoted rooting,while high concentration inhibited rooting,and rooting rate of 1/2MS reached 100% at 30 d.The sugar concentration also had an influence on rooting,which presented that rooting rate increased with the increase of sugar concentration,and the rooting rate was 99.02% at 40 g·L-1.NAA promoted rooting better than IBA,and the rooting rate reached 100% when NAA concentration was 0.10 mg·L-1.Therefor,the optimal medium for the root induction was 1/2MS+0.10 mg·L-1 NAA+40 g·L-1 sucrose.5.Transplanting substrate had a great effect on the survival rate of the transplanted plant,and the best transplanting substrate was perlite.But whether the tube plantlets having root or not had little effect on transplant survival rate. |
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