Transcriptome Data Analysis Of Spleen Of Grass Carp Infected By Reovirus

Grass carp(Ctenopharyngodon idella)was one of the important economical freshwater fish in China.However,hemorrhagic disease caused by grass carp reovirus(GCRV)results in a tremendous loss in the process of grass carp cultivation.Transcriptome analysis could provide a comprehensive understanding of the molecular mechanisms involved in specific biological processes and diseases for the resistance to reovirus infection of grass carp.In this study,the raw data from NCBI(accession number: SRA099702)were analyzed,in which,50 significant differentially expressed genes by routine transcriptome analysis and 84 notably differentially expressed genes by co-expression network method.KEGG analysis revealed that the pathway in hemorrhagic diseases in grass carp was similar to the influenza A induced pathway.The interferon-stimulated gene ISG15 and sacsin-like gene,which were up-regulated in data(SRA099702),were also up-regulated in data(SRP049081)from a similar assay.QPCR experiment was performed to validate these up-regulated genes.The ISG15 gene was shown to be the core gene in the co-expression network.The results would enhance our understanding of the antivirus system of grass carp infected by reovirus.